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Literature summary extracted from

  • Plaxton, W.C.; Storey, K.B.
    Purification and properties of aerobic and anoxic forms of pyruvate kinase from the hepatopancreas of the channelled whelk, Busycotypus canaliculatum (1985), Arch. Biochem. Biophys., 243, 195-205.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 asparagine activation Busycotypus canaliculatum
2.7.1.40 D-fructose 1,6-diphosphate activation Busycotypus canaliculatum
2.7.1.40 D-fructose 1,6-diphosphate stimulates aerobic isozyme more strongly than anoxic isozyme Busycotypus canaliculatum
2.7.1.40 D-fructose 1,6-diphosphate synergism with Asp, PK-aerobic, not PK-anoxic Busycotypus canaliculatum
2.7.1.40 additional information interacting effects of various activators and inhibitors Busycotypus canaliculatum

General Stability

EC Number General Stability Organism
2.7.1.40 0.5 M sucrose and 0.1 M KCl stabilize Busycotypus canaliculatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 ADP activators restore Busycotypus canaliculatum
2.7.1.40 ATP MgATP2- Busycotypus canaliculatum
2.7.1.40 MgATP2- activators restore activity Busycotypus canaliculatum
2.7.1.40 additional information interacting effects of various activators and inhibitors Busycotypus canaliculatum
2.7.1.40 phosphate activators restore Busycotypus canaliculatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
 additional information kinetic parameters Busycotypus canaliculatum
2.7.1.40 0.04 0.063 phosphoenolpyruvate in the absence of fructose 1,6-diphosphate Busycotypus canaliculatum
2.7.1.40 0.04 0.063 phosphoenolpyruvate in the presence of fructose 1,6-diphosphate Busycotypus canaliculatum
2.7.1.40 0.04 0.063 phosphoenolpyruvate pH 7.0, 20°C Busycotypus canaliculatum
2.7.1.40 0.2 0.35 ADP pH 7.0, 20°C Busycotypus canaliculatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+
-
 Busycotypus canaliculatum
2.7.1.40 Mg2+
-
 Busycotypus canaliculatum
2.7.1.40 Mn2+ can partially replace Mg2+ Busycotypus canaliculatum
2.7.1.40 additional information interacting effects of various activators and inhibitors Busycotypus canaliculatum
2.7.1.40 NH4+ requirement Busycotypus canaliculatum
2.7.1.40 NH4+ can replace K+ Busycotypus canaliculatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 71000
-
 4 * 71000, PK-aerobic, SDS-PAGE Busycotypus canaliculatum
2.7.1.40 72600
-
 4 * 72600, PK-anoxic, SDS-PAGE Busycotypus canaliculatum
2.7.1.40 286000
-
 PK-aerobic, gel filtration Busycotypus canaliculatum
2.7.1.40 299800
-
 PK-anoxic, gel filtration Busycotypus canaliculatum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Busycotypus canaliculatum
-
 channelled whelk, gastropod mollusc
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 aerobic and anoxic variants, i.e. isozymes PK-aerobic and PK-anoxic Busycotypus canaliculatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 hepatopancreas
-
 Busycotypus canaliculatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Busycotypus canaliculatum

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 4°C, in 25 mM imidazole-HCl buffer, pH 7.1, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 M sucrose and 0.04% NaN3, concentrated by dialysis against solid polyethylene glycol, at least 4 weeks, isozyme PK-aerobic Busycotypus canaliculatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Busycotypus canaliculatum ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 72600, PK-anoxic, SDS-PAGE Busycotypus canaliculatum
2.7.1.40 tetramer 4 * 71000, PK-aerobic, SDS-PAGE Busycotypus canaliculatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7
-
 broad Busycotypus canaliculatum