BRENDA - Enzyme Database

Purification and properties of aerobic and anoxic forms of pyruvate kinase from red muscle tissue of the channelled whelk, Busycotypus canaliculatum

Plaxton, W.C.; Storey, K.B.; Eur. J. Biochem. 143, 257-265 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
D-fructose 1,6-diphosphate
activation
Busycotypus canaliculatum
2.7.1.40
D-fructose 2,6-diphosphate
activation
Busycotypus canaliculatum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
2-oxoglutarate
not
Busycotypus canaliculatum
2.7.1.40
3-phosphoglycerate
not
Busycotypus canaliculatum
2.7.1.40
ADP
at high concentrations; fructose 1,6-diphosphate partially restores
Busycotypus canaliculatum
2.7.1.40
AMP
MgAMP
Busycotypus canaliculatum
2.7.1.40
arginine phosphate
allosteric inhibitor; fructose 1,6-diphosphate restores
Busycotypus canaliculatum
2.7.1.40
ATP
MgATP2-
Busycotypus canaliculatum
2.7.1.40
citrate
not
Busycotypus canaliculatum
2.7.1.40
glucose 6-phosphate
not
Busycotypus canaliculatum
2.7.1.40
isocitrate
not
Busycotypus canaliculatum
2.7.1.40
L-alanine
allosteric inhibitor; fructose 1,6-diphosphate restores
Busycotypus canaliculatum
2.7.1.40
L-lactate
-
Busycotypus canaliculatum
2.7.1.40
L-phenylalanine
alanine and fructose 1,6-diphosphate protect, kinetics; allosteric inhibitor; pH-dependent
Busycotypus canaliculatum
2.7.1.40
malate
not
Busycotypus canaliculatum
2.7.1.40
MgATP2-
allosteric inhibitor; fructose 1,6-diphosphate restores
Busycotypus canaliculatum
2.7.1.40
additional information
no inhibition by acetyl-CoA, NADP+, succinate, glycerol 1-phosphate, fructose 6-phosphate, D-octopine, meso-alanopine, NH4Cl, Arg, Gly, taurine, creatine phosphate
Busycotypus canaliculatum
2.7.1.40
oxaloacetate
not
Busycotypus canaliculatum
2.7.1.40
phosphate
not
Busycotypus canaliculatum
2.7.1.40
proline
allosteric inhibitor, fructose 1,6-diphosphate restores
Busycotypus canaliculatum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
additional information
-
additional information
kinetic properties
Busycotypus canaliculatum
2.7.1.40
0.2
0.35
ADP
pH 7.0, 20░C
Busycotypus canaliculatum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
K+
-
Busycotypus canaliculatum
2.7.1.40
Mg2+
-
Busycotypus canaliculatum
2.7.1.40
Mn2+
can partially replace Mg2+
Busycotypus canaliculatum
2.7.1.40
NH4+
can replace K+; requirement
Busycotypus canaliculatum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
64400
-
4 * 64400, PK-aerobic, SDS-PAGE
Busycotypus canaliculatum
2.7.1.40
232600
-
PK-anoxic, gel filtration
Busycotypus canaliculatum
2.7.1.40
237500
-
PK-aerobic, gel filtration
Busycotypus canaliculatum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.40
Busycotypus canaliculatum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.40
aerobic and anoxic variants, i.e. isozymes PK-aerobic and PK-anoxic
Busycotypus canaliculatum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
muscle
radular retractor muscle; red muscle
Busycotypus canaliculatum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.40
additional information
-
-
Busycotypus canaliculatum
2.7.1.40
368
-
isozyme PK-aerobic, pH 7.0, 20░C
Busycotypus canaliculatum
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.40
4░C, in 25 mM imidazole-HCl buffer, pH 7.1, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 M sucrose and 0.04% NaN3, concentrated by dialysis against solid polyethylene glycol, at least 4 weeks, isozyme PK-aerobic
Busycotypus canaliculatum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
-
641565
Busycotypus canaliculatum
ATP + pyruvate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.40
tetramer
4 * 64400, PK-aerobic, SDS-PAGE
Busycotypus canaliculatum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
additional information
-
pH-dependence of phosphoenolpyruvate affinity
Busycotypus canaliculatum
2.7.1.40
7
-
broad
Busycotypus canaliculatum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.40
6
8
about 70% of maximal activity at pH 6 and about half-maximal activity at pH 8
Busycotypus canaliculatum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.1.40
Busycotypus canaliculatum
isozyme PK-anoxic, isoelectric focusing
-
5.42
2.7.1.40
Busycotypus canaliculatum
isozyme PK-aerobic, isoelectric focusing
-
5.81
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
D-fructose 1,6-diphosphate
activation
Busycotypus canaliculatum
2.7.1.40
D-fructose 2,6-diphosphate
activation
Busycotypus canaliculatum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
2-oxoglutarate
not
Busycotypus canaliculatum
2.7.1.40
3-phosphoglycerate
not
Busycotypus canaliculatum
2.7.1.40
ADP
at high concentrations; fructose 1,6-diphosphate partially restores
Busycotypus canaliculatum
2.7.1.40
AMP
MgAMP
Busycotypus canaliculatum
2.7.1.40
arginine phosphate
allosteric inhibitor; fructose 1,6-diphosphate restores
Busycotypus canaliculatum
2.7.1.40
ATP
MgATP2-
Busycotypus canaliculatum
2.7.1.40
citrate
not
Busycotypus canaliculatum
2.7.1.40
glucose 6-phosphate
not
Busycotypus canaliculatum
2.7.1.40
isocitrate
not
Busycotypus canaliculatum
2.7.1.40
L-alanine
allosteric inhibitor; fructose 1,6-diphosphate restores
Busycotypus canaliculatum
2.7.1.40
L-lactate
-
Busycotypus canaliculatum
2.7.1.40
L-phenylalanine
alanine and fructose 1,6-diphosphate protect, kinetics; allosteric inhibitor; pH-dependent
Busycotypus canaliculatum
2.7.1.40
malate
not
Busycotypus canaliculatum
2.7.1.40
MgATP2-
allosteric inhibitor; fructose 1,6-diphosphate restores
Busycotypus canaliculatum
2.7.1.40
additional information
no inhibition by acetyl-CoA, NADP+, succinate, glycerol 1-phosphate, fructose 6-phosphate, D-octopine, meso-alanopine, NH4Cl, Arg, Gly, taurine, creatine phosphate
Busycotypus canaliculatum
2.7.1.40
oxaloacetate
not
Busycotypus canaliculatum
2.7.1.40
phosphate
not
Busycotypus canaliculatum
2.7.1.40
proline
allosteric inhibitor, fructose 1,6-diphosphate restores
Busycotypus canaliculatum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
additional information
-
additional information
kinetic properties
Busycotypus canaliculatum
2.7.1.40
0.2
0.35
ADP
pH 7.0, 20░C
Busycotypus canaliculatum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
K+
-
Busycotypus canaliculatum
2.7.1.40
Mg2+
-
Busycotypus canaliculatum
2.7.1.40
Mn2+
can partially replace Mg2+
Busycotypus canaliculatum
2.7.1.40
NH4+
can replace K+; requirement
Busycotypus canaliculatum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
64400
-
4 * 64400, PK-aerobic, SDS-PAGE
Busycotypus canaliculatum
2.7.1.40
232600
-
PK-anoxic, gel filtration
Busycotypus canaliculatum
2.7.1.40
237500
-
PK-aerobic, gel filtration
Busycotypus canaliculatum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
aerobic and anoxic variants, i.e. isozymes PK-aerobic and PK-anoxic
Busycotypus canaliculatum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
muscle
radular retractor muscle; red muscle
Busycotypus canaliculatum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.40
additional information
-
-
Busycotypus canaliculatum
2.7.1.40
368
-
isozyme PK-aerobic, pH 7.0, 20░C
Busycotypus canaliculatum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.40
4░C, in 25 mM imidazole-HCl buffer, pH 7.1, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 M sucrose and 0.04% NaN3, concentrated by dialysis against solid polyethylene glycol, at least 4 weeks, isozyme PK-aerobic
Busycotypus canaliculatum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
-
641565
Busycotypus canaliculatum
ATP + pyruvate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.40
tetramer
4 * 64400, PK-aerobic, SDS-PAGE
Busycotypus canaliculatum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
additional information
-
pH-dependence of phosphoenolpyruvate affinity
Busycotypus canaliculatum
2.7.1.40
7
-
broad
Busycotypus canaliculatum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.40
6
8
about 70% of maximal activity at pH 6 and about half-maximal activity at pH 8
Busycotypus canaliculatum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.1.40
Busycotypus canaliculatum
isozyme PK-anoxic, isoelectric focusing
-
5.42
2.7.1.40
Busycotypus canaliculatum
isozyme PK-aerobic, isoelectric focusing
-
5.81