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Literature summary extracted from

  • Schering, B.; Eigenbrodt, E.; Linder, D.; Schoner, W.
    Purification and properties of pyruvate kinase type M2 from rat lung (1982), Biochim. Biophys. Acta, 717, 337-347.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-fructose 1,6-diphosphate activation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 Ca2+
-
 Rattus norvegicus
2.7.1.40 cysteine fructose 1,6-diphosphate protects Rattus norvegicus
2.7.1.40 D-serine
-
 Rattus norvegicus
2.7.1.40 L-alanine fructose 1,6-diphosphate restores Rattus norvegicus
2.7.1.40 L-phenylalanine fructose 1,6-diphosphate protects Rattus norvegicus
2.7.1.40 additional information no phosphorylation Rattus norvegicus
2.7.1.40 threonine fructose 1,6-diphosphate protects Rattus norvegicus
2.7.1.40 tyrosine fructose 1,6-diphosphate partially protects Rattus norvegicus
2.7.1.40 valine fructose 1,6-diphosphate protects Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
 additional information kinetic parameters Rattus norvegicus
2.7.1.40 0.082 0.4 ADP pH 7.1, 37°C Rattus norvegicus
2.7.1.40 0.67
-
 GDP pH 7.1, 37°C Rattus norvegicus
2.7.1.40 1.25
-
 UDP pH 7.1, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+
-
 Rattus norvegicus
2.7.1.40 Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 224000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 lung M2-type isozyme Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 1341
-
 pH 7.1, 37°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Rattus norvegicus ATP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Rattus norvegicus CTP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Rattus norvegicus GTP + pyruvate
-
 ?
2.7.1.40 IDP + phosphoenolpyruvate less effective than ADP Rattus norvegicus ITP + pyruvate
-
 ?
2.7.1.40 UDP + phosphoenolpyruvate less effective than ADP Rattus norvegicus UTP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 62000-66000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.5
-
-
 Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.40 Rattus norvegicus
-
-
 5.8