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Literature summary extracted from

  • Crow, V.L.; Pritchard, G.G.
    Pyruvate kinase from Streptococcus lactis (1982), Methods Enzymol., 90, 165-170.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-fructose 1,6-diphosphate activation Lactococcus lactis
2.7.1.40 D-fructose 1,6-diphosphate requirement with Mg2+ Lactococcus lactis
2.7.1.40 D-fructose 1,6-diphosphate with Mn2+ Lactococcus lactis
2.7.1.40 D-fructose 6-phosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
2.7.1.40 D-glucose 6-phosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
2.7.1.40 D-ribose 5-phosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
2.7.1.40 D-tagatose 1,6-diphosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
2.7.1.40 D-tagatose 6-phosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
2.7.1.40 dihydroxyacetone phosphate activation Lactococcus lactis
2.7.1.40 dihydroxyacetone phosphate requirement with Mg2+ Lactococcus lactis
2.7.1.40 dihydroxyacetone phosphate with Mn2+ Lactococcus lactis
2.7.1.40 erythrose 4-phosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
2.7.1.40 glyceraldehyde 3-phosphate activation Lactococcus lactis
2.7.1.40 glyceraldehyde 3-phosphate requirement with Mg2+ Lactococcus lactis
2.7.1.40 glyceraldehyde 3-phosphate with Mn2+ Lactococcus lactis
2.7.1.40 phosphorylated hexoses activation Lactococcus lactis
2.7.1.40 phosphorylated hexoses requirement with Mg2+ Lactococcus lactis
2.7.1.40 phosphorylated hexoses with Mn2+ Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 AMP in a cooperative manner with ATP Lactococcus lactis
2.7.1.40 ATP in a cooperative manner with AMP Lactococcus lactis
2.7.1.40 phosphate strong Lactococcus lactis
2.7.1.40 sulfate
-
 Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.098 0.1 GDP
-
 Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 Co2+ activation Lactococcus lactis
2.7.1.40 divalent cation requirement Lactococcus lactis
2.7.1.40 K+ requirement Lactococcus lactis
2.7.1.40 K+ as good as NH4+ Lactococcus lactis
2.7.1.40 Mg2+ no activity in the absence of sugar phosphate activator Lactococcus lactis
2.7.1.40 Mn2+ best activator Lactococcus lactis
2.7.1.40 Mn2+ activation Lactococcus lactis
2.7.1.40 Mn2+ the activity of the sugar phosphate-activated enzyme is reduced to 50% if Mn2+ is omitted Lactococcus lactis
2.7.1.40 monovalent cation requirement Lactococcus lactis
2.7.1.40 NH4+ requirement Lactococcus lactis
2.7.1.40 NH4+ as good as K+ Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 235000
-
 equilibrium sedimentation Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Lactococcus lactis
-
-
-
2.7.1.40 Lactococcus lactis C10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
 Lactococcus lactis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Lactococcus lactis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Lactococcus lactis ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Lactococcus lactis C10 ATP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Lactococcus lactis GTP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Lactococcus lactis C10 GTP + pyruvate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 25
-
 assay at Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7.5
-
-
 Lactococcus lactis