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Literature summary extracted from

  • Tuominen, F.W.; Bernlohr, R.W.
    Pyruvate kinase of Bacillus licheniformis (1975), Methods Enzymol., 42C, 157-166.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 additional information the initial rate of catalysis is modulated by substrate activation by phosphoenolpyruvate and ADP, activation by AMP and inhibition by ATP, phosphate and carbamoyl phosphate Bacillus licheniformis

General Stability

EC Number General Stability Organism
2.7.1.40 labile enzyme is stabilized in the active state throughout purification by avoiding extensive dilution to less than 40 mg protein/ml, and by inclusion of a stabilizing ligand mixture of phosphoenolpyruvate, phosphate and Mg2+ in supporting buffers if dilution is unavoidable Bacillus licheniformis
2.7.1.40 MgATP2-, NAD+, NADH, 50% glycerol or sucrose partially stabilizes Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 ATP in the presence of Mg2+, not Mn2+, reversible by AMP Bacillus licheniformis
2.7.1.40 Carbamoyl phosphate
-
 Bacillus licheniformis
2.7.1.40 phosphate
-
 Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.2
-
 phosphoenolpyruvate pH 7.1, 30°C, in the presence of Mg2+, 7fold lower in the presence of Mn2+ Bacillus licheniformis
2.7.1.40 0.7
-
 ADP in the presence of Mg2+, pH 7.1, 30°C Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 Mg2+ requirement Bacillus licheniformis
2.7.1.40 Mn2+ requirement Bacillus licheniformis
2.7.1.40 additional information K-type allosteric properties only in the presence of Mg2+, not Mn2+ Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate Bacillus licheniformis allosteric enzyme ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate Bacillus licheniformis A-5 allosteric enzyme ATP + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Bacillus licheniformis
-
 A-5
-
2.7.1.40 Bacillus licheniformis A-5
-
 A-5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 partial Bacillus licheniformis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture vegetative Bacillus licheniformis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 210
-
 pH 7.1, 30°C Bacillus licheniformis

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 -20°C, in potassium phosphate buffer, pH 7, MgCl2, phosphoenolpyruvate and glycerol, 5 months Bacillus licheniformis
2.7.1.40 4°C, ammonium sulfate preparation, at least 5 months Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Bacillus licheniformis ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate allosteric enzyme Bacillus licheniformis ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Bacillus licheniformis A-5 ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate allosteric enzyme Bacillus licheniformis A-5 ATP + pyruvate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 30
-
 assay at Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7 7.4
-
 Bacillus licheniformis