BRENDA - Enzyme Database

Kinetic and regulatory properties of pyruvate kinase isozymes from flight muscle and fat body of the cockroach, Periplaneta americana

Storey, K.; J. Comp. Physiol. B 155, 339-345 (1985)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
D-fructose 1,6-diphosphate
activation; not; strong activation, fat body enzyme
Periplaneta americana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
arginine phosphate
flight muscle isozyme: weak; fructose 1,6-diphosphate restores
Periplaneta americana
2.7.1.40
citrate
fat body isozyme; weak, flight muscle isozyme
Periplaneta americana
2.7.1.40
L-alanine
weak; weak, flight muscle isozyme
Periplaneta americana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
0.04
0.063
phosphoenolpyruvate
-
Periplaneta americana
2.7.1.40
0.2
0.35
ADP
-
Periplaneta americana
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
K+
activation
Periplaneta americana
2.7.1.40
Mg2+
Km-value: 0.8 mM
Periplaneta americana
2.7.1.40
Mn2+
Km-value: 0.6 mM, flight muscle isozyme
Periplaneta americana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
235000
-
-
Periplaneta americana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.40
Periplaneta americana
-
cockroach
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.40
2 isozymes, in flight muscle and fat body
Periplaneta americana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
fat body
-
Periplaneta americana
-
2.7.1.40
muscle
flight muscle
Periplaneta americana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
-
641534
Periplaneta americana
ATP + pyruvate
-
-
-
?
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.1.40
Periplaneta americana
flight muscle isozyme
-
5.75
2.7.1.40
Periplaneta americana
fat body isozyme
-
6.15
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
D-fructose 1,6-diphosphate
activation; not; strong activation, fat body enzyme
Periplaneta americana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
arginine phosphate
flight muscle isozyme: weak; fructose 1,6-diphosphate restores
Periplaneta americana
2.7.1.40
citrate
fat body isozyme; weak, flight muscle isozyme
Periplaneta americana
2.7.1.40
L-alanine
weak; weak, flight muscle isozyme
Periplaneta americana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
0.04
0.063
phosphoenolpyruvate
-
Periplaneta americana
2.7.1.40
0.2
0.35
ADP
-
Periplaneta americana
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
K+
activation
Periplaneta americana
2.7.1.40
Mg2+
Km-value: 0.8 mM
Periplaneta americana
2.7.1.40
Mn2+
Km-value: 0.6 mM, flight muscle isozyme
Periplaneta americana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
235000
-
-
Periplaneta americana
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
2 isozymes, in flight muscle and fat body
Periplaneta americana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
fat body
-
Periplaneta americana
-
2.7.1.40
muscle
flight muscle
Periplaneta americana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
-
641534
Periplaneta americana
ATP + pyruvate
-
-
-
?
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.1.40
Periplaneta americana
flight muscle isozyme
-
5.75
2.7.1.40
Periplaneta americana
fat body isozyme
-
6.15