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Literature summary extracted from

  • Chrispeels, M.J.; Gaede, G.
    Purification and characterization of pyruvate kinase from the foot muscle of the cockle, Cardium tuberculatum (1985), Comp. Biochem. Physiol. B, 82, 162-172.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-fructose 1,6-diphosphate activation Acanthocardia tuberculata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 citrate
-
 Acanthocardia tuberculata
2.7.1.40 L-alanine phosphoenolpyruvate- and Mg2+-dependent Acanthocardia tuberculata
2.7.1.40 L-lactate
-
 Acanthocardia tuberculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.049 0.1 IDP cosubstrate phosphoenolpyruvate Acanthocardia tuberculata
2.7.1.40 0.2 0.35 ADP
-
 Acanthocardia tuberculata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ requirement Acanthocardia tuberculata
2.7.1.40 Mn2+ can partially replace Mg2+ Acanthocardia tuberculata
2.7.1.40 Mn2+ best activator Acanthocardia tuberculata
2.7.1.40 monovalent cation requirement Acanthocardia tuberculata
2.7.1.40 NH4+ requirement Acanthocardia tuberculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 70000
-
 4 * 70000 Acanthocardia tuberculata
2.7.1.40 282000
-
 gel filtration Acanthocardia tuberculata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate Acanthocardia tuberculata allosteric enzyme ATP + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Acanthocardia tuberculata
-
 cockle
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
 Acanthocardia tuberculata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 foot
-
 Acanthocardia tuberculata
-
2.7.1.40 muscle foot muscle Acanthocardia tuberculata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Acanthocardia tuberculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Acanthocardia tuberculata ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate allosteric enzyme Acanthocardia tuberculata ATP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Acanthocardia tuberculata GTP + pyruvate
-
 ?
2.7.1.40 IDP + phosphoenolpyruvate less effective than ADP Acanthocardia tuberculata ITP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 70000 Acanthocardia tuberculata