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Literature summary extracted from
- Purification and properties of pyruvate kinase type M1 from bovine brain (1989), Int. J. Biochem., 21, 1053-1060.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.40 | phosphate | activation | Bos taurus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.40 | ATP | above 6 mM | Bos taurus | |
| 2.7.1.40 | L-phenylalanine | fructose 1,6-diphosphate protects | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.1.40 | 0.04 | 0.063 | phosphoenolpyruvate | - |
Bos taurus | |
| 2.7.1.40 | 0.2 | 0.35 | ADP | - |
Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.40 | 57000 | - |
x * 57000, SDS-PAGE | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.1.40 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.1.40 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.1.40 | brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.40 | additional information | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.40 | ADP + phosphoenolpyruvate | - |
Bos taurus | ATP + pyruvate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.1.40 | ? | x * 57000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.40 | 25 | - |
assay at | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.40 | 6.8 | 7.2 | - |
Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.40 | ATP | activation | Bos taurus | |
| 2.7.1.40 | ATP | below 4 mM, inhibits above 5 mM | Bos taurus | |
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