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Literature summary extracted from

  • Wu, H.B.; Turpin, D.H.
    Purification and characteization of pyruvate kinase from the green alga Chlamydomonas reinhardtii (1992), J. Phycol., 28, 472-481.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-Ribulose 1,5-diphosphate activation Chlamydomonas reinhardtii
2.7.1.40 dihydroxyacetone phosphate activation Chlamydomonas reinhardtii
2.7.1.40 glyceraldehyde 3-phosphate activation Chlamydomonas reinhardtii
2.7.1.40 ribulose 5-phosphate activation Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 2,3-diphosphoglycerate
-
 Chlamydomonas reinhardtii
2.7.1.40 ATP
-
 Chlamydomonas reinhardtii
2.7.1.40 citrate
-
 Chlamydomonas reinhardtii
2.7.1.40 isocitrate
-
 Chlamydomonas reinhardtii
2.7.1.40 L-glutamate dihydroxyacetone phosphate reverses Chlamydomonas reinhardtii
2.7.1.40 malate
-
 Chlamydomonas reinhardtii
2.7.1.40 N-ethylmaleimide
-
 Chlamydomonas reinhardtii
2.7.1.40 oxalate
-
 Chlamydomonas reinhardtii
2.7.1.40 Phosphoglycolate
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.15 0.16 phosphoenolpyruvate
-
 Chlamydomonas reinhardtii
2.7.1.40 0.18
-
 phosphoenolpyruvate cosubstrate ADP Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ activation Chlamydomonas reinhardtii
2.7.1.40 K+ strong Chlamydomonas reinhardtii
2.7.1.40 Mg2+ requirement Chlamydomonas reinhardtii
2.7.1.40 Mg2+ fulfills absolute requirement for divalent cation Chlamydomonas reinhardtii
2.7.1.40 Mn2+ requirement Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 57000
-
 4 * 57000, SDS-PAGE Chlamydomonas reinhardtii
2.7.1.40 224000
-
 native molecular mass Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 partial Chlamydomonas reinhardtii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.40 ATP + pyruvate = ADP + phosphoenolpyruvate mechanism Chlamydomonas reinhardtii
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Chlamydomonas reinhardtii ATP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Chlamydomonas reinhardtii CTP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate reaction at 25% the rate of ADP Chlamydomonas reinhardtii CTP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Chlamydomonas reinhardtii GTP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate reaction at 55% the rate of ADP Chlamydomonas reinhardtii GTP + pyruvate
-
 ?
2.7.1.40 IDP + phosphoenolpyruvate less effective than ADP Chlamydomonas reinhardtii ITP + pyruvate
-
 ?
2.7.1.40 IDP + phosphoenolpyruvate reaction at 53% the rate of ADP Chlamydomonas reinhardtii ITP + pyruvate
-
 ?
2.7.1.40 UDP + phosphoenolpyruvate less effective than ADP Chlamydomonas reinhardtii UTP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 57000, SDS-PAGE Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 additional information
-
 heat-stable enzyme Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.8
-
-
 Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 AMP activation Chlamydomonas reinhardtii