BRENDA - Enzyme Database

Purification and characteization of pyruvate kinase from the green alga Chlamydomonas reinhardtii

Wu, H.B.; Turpin, D.H.; J. Phycol. 28, 472-481 (1992)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
D-Ribulose 1,5-diphosphate
activation
Chlamydomonas reinhardtii
2.7.1.40
dihydroxyacetone phosphate
activation
Chlamydomonas reinhardtii
2.7.1.40
glyceraldehyde 3-phosphate
activation
Chlamydomonas reinhardtii
2.7.1.40
ribulose 5-phosphate
activation
Chlamydomonas reinhardtii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
2,3-diphosphoglycerate
-
Chlamydomonas reinhardtii
2.7.1.40
ATP
-
Chlamydomonas reinhardtii
2.7.1.40
citrate
-
Chlamydomonas reinhardtii
2.7.1.40
isocitrate
-
Chlamydomonas reinhardtii
2.7.1.40
L-glutamate
dihydroxyacetone phosphate reverses
Chlamydomonas reinhardtii
2.7.1.40
malate
-
Chlamydomonas reinhardtii
2.7.1.40
N-ethylmaleimide
-
Chlamydomonas reinhardtii
2.7.1.40
oxalate
-
Chlamydomonas reinhardtii
2.7.1.40
Phosphoglycolate
-
Chlamydomonas reinhardtii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
0.15
0.16
phosphoenolpyruvate
-
Chlamydomonas reinhardtii
2.7.1.40
0.18
-
phosphoenolpyruvate
cosubstrate ADP
Chlamydomonas reinhardtii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
K+
activation; strong
Chlamydomonas reinhardtii
2.7.1.40
Mg2+
fulfills absolute requirement for divalent cation; requirement
Chlamydomonas reinhardtii
2.7.1.40
Mn2+
requirement
Chlamydomonas reinhardtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
57000
-
4 * 57000, SDS-PAGE
Chlamydomonas reinhardtii
2.7.1.40
224000
-
native molecular mass
Chlamydomonas reinhardtii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.40
Chlamydomonas reinhardtii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.40
partial
Chlamydomonas reinhardtii
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.40
ATP + pyruvate = ADP + phosphoenolpyruvate
mechanism
Chlamydomonas reinhardtii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
cell culture
-
Chlamydomonas reinhardtii
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.40
additional information
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
best nucleoside diphosphate substrate
641516
Chlamydomonas reinhardtii
ATP + pyruvate
-
-
-
?
2.7.1.40
CDP + phosphoenolpyruvate
less effective than ADP
641516
Chlamydomonas reinhardtii
CTP + pyruvate
-
-
-
?
2.7.1.40
CDP + phosphoenolpyruvate
reaction at 25% the rate of ADP
641516
Chlamydomonas reinhardtii
CTP + pyruvate
-
-
-
?
2.7.1.40
GDP + phosphoenolpyruvate
less effective than ADP
641516
Chlamydomonas reinhardtii
GTP + pyruvate
-
-
-
?
2.7.1.40
GDP + phosphoenolpyruvate
reaction at 55% the rate of ADP
641516
Chlamydomonas reinhardtii
GTP + pyruvate
-
-
-
?
2.7.1.40
IDP + phosphoenolpyruvate
less effective than ADP
641516
Chlamydomonas reinhardtii
ITP + pyruvate
-
-
-
?
2.7.1.40
IDP + phosphoenolpyruvate
reaction at 53% the rate of ADP
641516
Chlamydomonas reinhardtii
ITP + pyruvate
-
-
-
?
2.7.1.40
UDP + phosphoenolpyruvate
less effective than ADP
641516
Chlamydomonas reinhardtii
UTP + pyruvate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.40
tetramer
4 * 57000, SDS-PAGE
Chlamydomonas reinhardtii
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
2.7.1.40
additional information
-
heat-stable enzyme
Chlamydomonas reinhardtii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
6.8
-
-
Chlamydomonas reinhardtii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.40
AMP
activation
Chlamydomonas reinhardtii
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
D-Ribulose 1,5-diphosphate
activation
Chlamydomonas reinhardtii
2.7.1.40
dihydroxyacetone phosphate
activation
Chlamydomonas reinhardtii
2.7.1.40
glyceraldehyde 3-phosphate
activation
Chlamydomonas reinhardtii
2.7.1.40
ribulose 5-phosphate
activation
Chlamydomonas reinhardtii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.40
AMP
activation
Chlamydomonas reinhardtii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
2,3-diphosphoglycerate
-
Chlamydomonas reinhardtii
2.7.1.40
ATP
-
Chlamydomonas reinhardtii
2.7.1.40
citrate
-
Chlamydomonas reinhardtii
2.7.1.40
isocitrate
-
Chlamydomonas reinhardtii
2.7.1.40
L-glutamate
dihydroxyacetone phosphate reverses
Chlamydomonas reinhardtii
2.7.1.40
malate
-
Chlamydomonas reinhardtii
2.7.1.40
N-ethylmaleimide
-
Chlamydomonas reinhardtii
2.7.1.40
oxalate
-
Chlamydomonas reinhardtii
2.7.1.40
Phosphoglycolate
-
Chlamydomonas reinhardtii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
0.15
0.16
phosphoenolpyruvate
-
Chlamydomonas reinhardtii
2.7.1.40
0.18
-
phosphoenolpyruvate
cosubstrate ADP
Chlamydomonas reinhardtii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
K+
activation; strong
Chlamydomonas reinhardtii
2.7.1.40
Mg2+
fulfills absolute requirement for divalent cation; requirement
Chlamydomonas reinhardtii
2.7.1.40
Mn2+
requirement
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
57000
-
4 * 57000, SDS-PAGE
Chlamydomonas reinhardtii
2.7.1.40
224000
-
native molecular mass
Chlamydomonas reinhardtii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
partial
Chlamydomonas reinhardtii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
cell culture
-
Chlamydomonas reinhardtii
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.40
additional information
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
best nucleoside diphosphate substrate
641516
Chlamydomonas reinhardtii
ATP + pyruvate
-
-
-
?
2.7.1.40
CDP + phosphoenolpyruvate
less effective than ADP
641516
Chlamydomonas reinhardtii
CTP + pyruvate
-
-
-
?
2.7.1.40
CDP + phosphoenolpyruvate
reaction at 25% the rate of ADP
641516
Chlamydomonas reinhardtii
CTP + pyruvate
-
-
-
?
2.7.1.40
GDP + phosphoenolpyruvate
less effective than ADP
641516
Chlamydomonas reinhardtii
GTP + pyruvate
-
-
-
?
2.7.1.40
GDP + phosphoenolpyruvate
reaction at 55% the rate of ADP
641516
Chlamydomonas reinhardtii
GTP + pyruvate
-
-
-
?
2.7.1.40
IDP + phosphoenolpyruvate
less effective than ADP
641516
Chlamydomonas reinhardtii
ITP + pyruvate
-
-
-
?
2.7.1.40
IDP + phosphoenolpyruvate
reaction at 53% the rate of ADP
641516
Chlamydomonas reinhardtii
ITP + pyruvate
-
-
-
?
2.7.1.40
UDP + phosphoenolpyruvate
less effective than ADP
641516
Chlamydomonas reinhardtii
UTP + pyruvate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.40
tetramer
4 * 57000, SDS-PAGE
Chlamydomonas reinhardtii
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
2.7.1.40
additional information
-
heat-stable enzyme
Chlamydomonas reinhardtii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
6.8
-
-
Chlamydomonas reinhardtii