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Literature summary extracted from

  • Baysdorfer, C.; Kremer, D.F.; Sicher, R.C.
    Partial purification and characterization of fructokinase activity from barley leaves (1989), J. Plant Physiol., 134, 156-161.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.4 D-fructose severe inhibition with fructokinase-Ia and-Ib, slight with fructokinase II; substrate inhibition Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.006
-
 D-fructose fructokinase Ib, pH 7.6, 25°C Hordeum vulgare
2.7.1.4 0.012
-
 D-fructose fructokinase II, pH 7.6, 25°C Hordeum vulgare
2.7.1.4 0.12
-
 D-fructose fructokinase Ia, pH 7.6, 25°C Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.4 soluble
-
 Hordeum vulgare
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.4 K+ stimulates Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.4 37000
-
 fructokinase-Ia, and fructokinase-1b, gel filtration Hordeum vulgare
2.7.1.4 73000
-
 fructokinase II, gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.4 ATP + D-fructose Hordeum vulgare
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Hordeum vulgare
-
 L. cv. Brant, barley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.4 partial, 3 forms: fructokinase-Ia,-Ib,-II Hordeum vulgare

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.4 51.8
-
 fructokinase Ia and Ib Hordeum vulgare
2.7.1.4 60.3
-
 fructokinase II Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose
-
 Hordeum vulgare ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 UTP + D-fructose
-
 Hordeum vulgare UDP + D-fructose 6-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.4 25
-
 assay at Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 8
-
-
 Hordeum vulgare