Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Copeland, L.; Morell, M.
    Hexose kinases from the plant cytosolic fraction of soybean nodules (1985), Plant Physiol., 79, 114-117.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.4 D-fructose above 0.4 mM at pH 8.2, no substrate inhibition at pH 6.6; substrate inhibition Glycine max
2.7.1.4 MgADP-
-
 Glycine max
2.7.1.4 Mn2+ can partially replace Mg2+ in activation, inhibition above 0.5 mM at 1 mM ATP Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.055
-
 D-fructose 30°C, pH 6.6 Glycine max
2.7.1.4 0.077
-
 D-fructose 30°C, pH 8.2 Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.4 cytoplasm
-
 Glycine max 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.4 Cs+ slight stimulation Glycine max
2.7.1.4 K+ monovalent cation required for maximal activity, K+ most effective Glycine max
2.7.1.4 Li+ slight stimulation Glycine max
2.7.1.4 Mg2+ at 1 mM ATP maximal activity with 1.5 mM MgCl2, higher concentrations inhibit Glycine max
2.7.1.4 Mg2+ Km: 0.095 mM for MgATP2- Glycine max
2.7.1.4 Mn2+ can partially replace Mg2+ in activation Glycine max
2.7.1.4 Mn2+ inhibition above 0.5 mM at 1 mM ATP Glycine max
2.7.1.4 Na+ slight stimulation Glycine max
2.7.1.4 NH4+ can partially replace K+ in activation Glycine max
2.7.1.4 Rb+ can partially replace K+ in activation Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.4 84000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.4 ATP + D-fructose Glycine max
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Glycine max
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.4 nodule
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.4 11.2
-
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose
-
 Glycine max ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ATP + D-fructose specific for fructose Glycine max ADP + D-fructose 6-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.4 30
-
 assay at Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 8.2
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.4 7.4 9.3 more than 90% of activity maximum at pH 7.4 and pH 9.3 Glycine max