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Literature summary extracted from

  • Anderson, R.L.; Sapico, V.L.
    D-fructose (D-mannose) kinase (1975), Methods Enzymol., 42C, 39-43.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.4
-
 D-fructose pH 6.9, 25°C Leuconostoc mesenteroides
2.7.1.4 0.4
-
 D-mannose pH 6.9, 25°C Leuconostoc mesenteroides
2.7.1.7 0.4
-
 D-fructose pH 6.9, 25°C Leuconostoc mesenteroides
2.7.1.7 0.4
-
 D-mannose pH 6.9, 25°C Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.4 47000
-
 sucrose density gradient centrifugation Leuconostoc mesenteroides
2.7.1.7 47000
-
 sucrose density gradient centrifugation Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.4 ATP + D-fructose Leuconostoc mesenteroides
-
 ADP + D-fructose 6-phosphate
-
 ?
2.7.1.7 ATP + D-fructose Leuconostoc mesenteroides enzyme initiates the metabolism of D-fructose and D-mannose ADP + D-fructose 6-phosphate
-
 ?
2.7.1.7 ATP + D-mannose Leuconostoc mesenteroides enzyme initiates the metabolism of D-fructose and D-mannose ADP + D-mannose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Leuconostoc mesenteroides
-
-
-
2.7.1.7 Leuconostoc mesenteroides
-
 a single enzyme catalyzes phosphorylation of D-fructose and D-mannose
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.4
-
 Leuconostoc mesenteroides
2.7.1.7
-
 Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.4 76
-
-
 Leuconostoc mesenteroides
2.7.1.7 76
-
-
 Leuconostoc mesenteroides

Storage Stability

EC Number Storage Stability Organism
2.7.1.4 0°C, 0.2 (NH4)2SO4, pH 7.0, stable for several months Leuconostoc mesenteroides
2.7.1.7 0°C, 0.2 M (NH4)2SO4, pH 7.0, stable for several months Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose
-
 Leuconostoc mesenteroides ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ATP + D-mannose D-fructose and D-mannose phosphorylated at equivalent rates Leuconostoc mesenteroides ADP + D-mannose 6-phosphate
-
 ?
2.7.1.7 ATP + D-fructose D-mannose and D-fructose phosphorylated equally well Leuconostoc mesenteroides ADP + D-fructose 6-phosphate
-
 ?
2.7.1.7 ATP + D-fructose enzyme initiates the metabolism of D-fructose and D-mannose Leuconostoc mesenteroides ADP + D-fructose 6-phosphate
-
 ?
2.7.1.7 ATP + D-glucose no activity with D-glucose or L-glucose Leuconostoc mesenteroides ADP + D-glucose 6-phosphate
-
 ?
2.7.1.7 ATP + D-mannose enzyme initiates the metabolism of D-fructose and D-mannose Leuconostoc mesenteroides ADP + D-mannose 6-phosphate
-
 ?
2.7.1.7 ATP + D-mannose D-mannose and D-fructose phosphorylated equally well Leuconostoc mesenteroides ADP + mannose 6-phosphate D-mannose 6-phosphate ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 6.9
-
 D-mannose Leuconostoc mesenteroides
2.7.1.4 7 8.5 D-fructose Leuconostoc mesenteroides
2.7.1.7 6.9
-
 reaction with D-mannose Leuconostoc mesenteroides
2.7.1.7 7 8.5 reaction with D-fructose Leuconostoc mesenteroides