Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Theze, J.; Kleidman, L.; Saint Girons, I.
    Homoserine kinase from Escherichia coli K-12: properties, inhibition by L-threonine, and regulation of biosynthesis (1974), J. Bacteriol., 118, 577-581.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.39 NH4+ 30% of the activity obtained with 0.5 M K+ Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.39 alpha-amino-beta-hydroxy valeric acid
-
 Escherichia coli
2.7.1.39 L-2-aminobutyric acid 40 mM, 50% inhibition Escherichia coli
2.7.1.39 L-Thr
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.39 0.3
-
 ATP pH 7.8, 27°C Escherichia coli
2.7.1.39 0.3
-
 L-homoserine pH 7.8, 27°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.39 K+ activates Escherichia coli
2.7.1.39 Mg2+ absolute requirement, optimal activity between 10 mM and 20 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.39 60000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.39 Escherichia coli
-
 K-12
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.39 ATP + L-homoserine
-
 Escherichia coli ADP + O-phospho-L-homoserine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.39 57
-
 half-life is 10 min in absence of L-Thr ot L-homoserine Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.39 7.8
-
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.39 1
-
 L-Thr pH 7.8, 27°C Escherichia coli