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Literature summary extracted from

  • Chu, X.; Liu, X.; Yau, M.; Leung, Y.C.; Li, D.
    Expression and purification of Arg196 and Lys272 mutants of mevalonate kinase from Methanococcus jannaschii (2003), Protein Expr. Purif., 30, 210-218.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.36 K272A 18% of wild-type activity Methanocaldococcus jannaschii
2.7.1.36 K272R 18% of wild-type activity Methanocaldococcus jannaschii
2.7.1.36 R196L 5% of wild-type activity Methanocaldococcus jannaschii
2.7.1.36 R196V 60% of wild-type activity Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.11
-
 mevalonate pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 0.25
-
 mevalonate pH 7.5, 34°C, K272R mutant mevalonate kinase Methanocaldococcus jannaschii
2.7.1.36 0.51
-
 mevalonate pH 7.5, 34°C, K272A mutant mevalonate kinase Methanocaldococcus jannaschii
2.7.1.36 1.18
-
 ATP pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 1.22
-
 ATP pH 7.5, 34°C, K272R mutant mevalonate kinase Methanocaldococcus jannaschii
2.7.1.36 1.43
-
 mevalonate pH 7.5, 34°C, R196L mutant mevalonate kinase Methanocaldococcus jannaschii
2.7.1.36 1.54
-
 ATP pH 7.5, 34°C, R196L mutant mevalonate kinase Methanocaldococcus jannaschii
2.7.1.36 2.95
-
 ATP pH 7.5, 34°C, K272A mutant mevalonate kinase Methanocaldococcus jannaschii
2.7.1.36 2.98
-
 ATP pH 7.5, 34°C, R196Q mutant mevalonate kinase Methanocaldococcus jannaschii
2.7.1.36 3
-
 ATP pH 7.5, 34°C, R196V mutant mevalonate kinase Methanocaldococcus jannaschii
2.7.1.36 4.03
-
 mevalonate pH 7.5, 34°C, R196V mutant mevalonate kinase Methanocaldococcus jannaschii
2.7.1.36 9.28
-
 mevalonate pH 7.5, 34°C, R196Q mutant mevalonate kinase Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Methanocaldococcus jannaschii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + mevalonate
-
 Methanocaldococcus jannaschii ADP + phosphomevalonate
-
 ?