Literature summary extracted from
Chu, X.; Li, D.
Cloning, expression, and purification of His-tagged rat mevalonate kinase (2003), Protein Expr. Purif., 27, 165-170.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.1.36 |
expression of His-tagged mevalonate kinase in Escherichia coli |
Rattus norvegicus |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.7.1.36 |
0.035 |
- |
(R,S)-mevalonate |
pH 7.5, 25°C, recombinant mevalonate kinase |
Rattus norvegicus |
|
2.7.1.36 |
0.95 |
- |
ATP |
pH 7.5, 25°C, recombinant mevalonate kinase |
Rattus norvegicus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.1.36 |
42000 |
- |
2 * 42000, recombinant mevalonate kinase, SDS-PAGE |
Rattus norvegicus |
2.7.1.36 |
84000 |
- |
recombinant mevalonate kinase, gel filtration |
Rattus norvegicus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.1.36 |
Rattus norvegicus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.1.36 |
recombinant His-tagged mevalonate kinase, hiTrap column |
Rattus norvegicus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.7.1.36 |
32.7 |
- |
recombinant mevalonate kinase |
Rattus norvegicus |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.7.1.36 |
4°C, 20 mM potassium phosphate, pH 7.5, 5% glycerol, 5 mM 2-mercaptoethanol, 1 month, no loss of activity |
Rattus norvegicus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.1.36 |
ATP + mevalonate |
- |
Rattus norvegicus |
ADP + phosphomevalonate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.1.36 |
dimer |
2 * 42000, recombinant mevalonate kinase, SDS-PAGE |
Rattus norvegicus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.7.1.36 |
7 |
8 |
recombinant mevalonate kinase |
Rattus norvegicus |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
2.7.1.36 |
6 |
9 |
at least 80% of maximal activity over the whole range, recombinant mevalonate kinase |
Rattus norvegicus |