BRENDA - Enzyme Database

Post-translational regulation of mevalonate kinase by intermediates of the cholesterol and nonsterol isoprene biosynthetic pathways

Hinson, D.D.; Chambliss, K.L.; Toth, M.J.; Tanaka, R.D.; Gibson, K.M.; J. Lipid Res. 38, 2216-2223 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.36
expression in Escherichia coli
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.36
3,3-dimethylallyl diphosphate
-
Homo sapiens
2.7.1.36
dolichol phosphate
-
Homo sapiens
2.7.1.36
farnesol
-
Homo sapiens
2.7.1.36
farnesyl diphosphate
-
Homo sapiens
2.7.1.36
geranyl diphosphate
-
Homo sapiens
2.7.1.36
geranylgeranyl diphosphate
-
Homo sapiens
2.7.1.36
isopentenyl diphosphate
-
Homo sapiens
2.7.1.36
additional information
not inhibited by R-mevalonate 5-diphosphate, geraniol and dolichol up to 0.084, 0.226 and 0.68 mM, respectively
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.36
0.15
-
(R,S)-mevalonate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.44
-
ATP
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.36
42500
-
x * 42500, recombinant mevalonate kinase, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.36
ATP + mevalonate
Homo sapiens
part of the cholesterol and nonsterol isoprene biosynthetic pathway
ADP + phosphomevalonate
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.36
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.36
recombinant mevalonat kinase-glutathione S-transferase fusion protein, glutathione Sepharose beads
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.36
ATP + mevalonate
-
641447
Homo sapiens
ADP + phosphomevalonate
-
641447
Homo sapiens
?
2.7.1.36
ATP + mevalonate
part of the cholesterol and nonsterol isoprene biosynthetic pathway
641447
Homo sapiens
ADP + phosphomevalonate
-
641447
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.36
?
x * 42500, recombinant mevalonate kinase, SDS-PAGE
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.36
0.000104
-
farnesyl diphosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.016
-
isopentenyl diphosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.02
-
3,3-dimethylallyl diphosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.059
-
geranylgeranyl diphosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.072
-
farnesol
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.083
-
dolichol phosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.116
-
geranyl diphosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.36
expression in Escherichia coli
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.36
3,3-dimethylallyl diphosphate
-
Homo sapiens
2.7.1.36
dolichol phosphate
-
Homo sapiens
2.7.1.36
farnesol
-
Homo sapiens
2.7.1.36
farnesyl diphosphate
-
Homo sapiens
2.7.1.36
geranyl diphosphate
-
Homo sapiens
2.7.1.36
geranylgeranyl diphosphate
-
Homo sapiens
2.7.1.36
isopentenyl diphosphate
-
Homo sapiens
2.7.1.36
additional information
not inhibited by R-mevalonate 5-diphosphate, geraniol and dolichol up to 0.084, 0.226 and 0.68 mM, respectively
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.36
0.000104
-
farnesyl diphosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.016
-
isopentenyl diphosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.02
-
3,3-dimethylallyl diphosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.059
-
geranylgeranyl diphosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.072
-
farnesol
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.083
-
dolichol phosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.116
-
geranyl diphosphate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.36
0.15
-
(R,S)-mevalonate
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
2.7.1.36
0.44
-
ATP
pH 7.0, 25C, recombinant mevalonate kinase
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.36
42500
-
x * 42500, recombinant mevalonate kinase, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.36
ATP + mevalonate
Homo sapiens
part of the cholesterol and nonsterol isoprene biosynthetic pathway
ADP + phosphomevalonate
-
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.36
recombinant mevalonat kinase-glutathione S-transferase fusion protein, glutathione Sepharose beads
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.36
ATP + mevalonate
-
641447
Homo sapiens
ADP + phosphomevalonate
-
641447
Homo sapiens
?
2.7.1.36
ATP + mevalonate
part of the cholesterol and nonsterol isoprene biosynthetic pathway
641447
Homo sapiens
ADP + phosphomevalonate
-
641447
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.36
?
x * 42500, recombinant mevalonate kinase, SDS-PAGE
Homo sapiens