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Literature summary extracted from

  • Soong Lee, C.; O'Sullivan, W.J.
    An improved purification procedure, an alternative assay and activation of mevalonate kinase by ATP (1983), Biochim. Biophys. Acta, 747, 215-224.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.36 arsenate hyperbolic activation curve, 13% activation at 25 mM, 87% activation at 500 mM Sus scrofa
2.7.1.36 ATP4- most effective activator, maximal activation at 8 mM Sus scrofa
2.7.1.36 phosphate 50% activation at 100 mM Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 Mn2+ inhibition above 2.5 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.68
-
ATP pH 6.5, 30°C, at 1 mM Mg2+ Sus scrofa
2.7.1.36 0.68
-
ATP ATP in form of MgATP2- Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.36 Ba2+ can partially replace Mg2+ in activation Rattus norvegicus
2.7.1.36 Ba2+ can partially replace Mg2+ in activation Sus scrofa
2.7.1.36 Ba2+ at 5 mM 10% of activity with Mg2+ Sus scrofa
2.7.1.36 Ca2+ can partially replace Mn2+ in activation Rattus norvegicus
2.7.1.36 Ca2+ can partially replace Mn2+ in activation Sus scrofa
2.7.1.36 Ca2+ 71% of activity with Mg2+ Sus scrofa
2.7.1.36 Mg2+ presence of free ATP4- results in a biphasic Lineweaver-Burk plot with 2 apparent Km-values for MgATP2-: 0.14 mM and 0.006 mM Rattus norvegicus
2.7.1.36 Mg2+ presence of free ATP4- results in a biphasic Lineweaver-Burk plot with 2 apparent Km-values for MgATP2-: 0.14 mM and 0.006 mM Sus scrofa
2.7.1.36 Mn2+ can partially replace Mg2+ in activation, maximal activation at 2.5 mM, inhibition above Sus scrofa
2.7.1.36 Mn2+ most active at low concentrations Sus scrofa
2.7.1.36 Sr2+ can partially replace Mg2+ in activation, 8% of activity with Mg2+ at 5 mM Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 52000
-
2 * 52000, SDS-PAGE Sus scrofa
2.7.1.36 104000
-
gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Rattus norvegicus
-
-
-
2.7.1.36 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 ammonium sulfate, Blue-Sepharose, DEAE-Trisacryl, ATP-agarose, Sephadex G-150 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.36 liver
-
Rattus norvegicus
-
2.7.1.36 liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.36 additional information
-
assay procedure Sus scrofa
2.7.1.36 30.4
-
-
Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
Sus scrofa ADP + (R)-5-phosphomevalonate
-
?
2.7.1.36 ATP + mevalonate ATP is the most effective phosphate donor Rattus norvegicus ADP + phosphomevalonate
-
?
2.7.1.36 ATP + mevalonate ATP is the most effective phosphate donor Sus scrofa ADP + phosphomevalonate
-
ir
2.7.1.36 CTP + mevalonate 18% of activity with ATP Sus scrofa CDP + phosphomevalonate
-
?
2.7.1.36 dATP + mevalonate 67% of the activity with ATP Sus scrofa dADP + phosphomevalonate
-
?
2.7.1.36 dCTP + mevalonate 17% of the activity with ATP Sus scrofa dCDP + phosphomevalonate
-
?
2.7.1.36 dGTP + mevalonate 17% of the activity with ATP Sus scrofa dGDP + phosphomevalonate
-
?
2.7.1.36 GTP + mevalonate 18% of the activity with ATP Sus scrofa GDP + phosphomevalonate
-
?
2.7.1.36 ITP + mevalonate 88% of activity with ATP Sus scrofa IDP + phosphomevalonate
-
?
2.7.1.36 TTP + mevalonate 15% of the activity with ATP Sus scrofa TDP + phosphomevalonate
-
?
2.7.1.36 UTP + mevalonate 61% of the activity with ATP Sus scrofa UDP + phosphomevalonate
-
?
2.7.1.36 XTP + mevalonate 27% of the activity with ATP Sus scrofa XDP + phosphomevalonate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 dimer
-
Rattus norvegicus
2.7.1.36 dimer 2 * 52000, SDS-PAGE Sus scrofa