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Literature summary extracted from

  • Markley, K.; Smallan, E.
    Mevalonic kinase in rabbit liver (1961), Biochim. Biophys. Acta, 47, 327-335.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.36 cysteine or to a lesser extent other SH-compounds required Oryctolagus cuniculus
2.7.1.36 phosphate required for activity Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.7.1.36 almost complete loss of activity after dialysis against 1000 volumes of water, 2-mercaptoethanol, EDTA or glutathione Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 p-chloromercuribenzoate activity can be almost completely restored by incubation with an excess of cysteine Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 5.1
-
 DL-mevalonate pH 7.0, 35°C Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.36 Mg2+ activates Oryctolagus cuniculus
2.7.1.36 Mg2+ Mn2+ or Mg2+ required Oryctolagus cuniculus
2.7.1.36 Mg2+ most active divalent cation Oryctolagus cuniculus
2.7.1.36 Mn2+ divalent cation required for activity Oryctolagus cuniculus
2.7.1.36 Mn2+ maximal activation at approx. 0.0013 mM Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 ammonium sulfate, protamine sulfate, ammonium sulfate, DEAE-cellulose Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.36 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.36 0.11
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.36 -20°C, 50% loss of activity after 1 month Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + mevalonate ATP is the most effective phosphate donor Oryctolagus cuniculus ADP + phosphomevalonate
-
 ?
2.7.1.36 ATP + mevalonate no activity with ADP, GTP and UTP Oryctolagus cuniculus ADP + phosphomevalonate
-
 ?
2.7.1.36 ITP + mevalonate 36% of activity with ATP Oryctolagus cuniculus IDP + phosphomevalonate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.36 35 37 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.36 7 8
-
 Oryctolagus cuniculus