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Literature summary extracted from

  • Karawya, E.; Fonda, M.L.
    Physical and kinetic properties of sheep liver pyridoxine kinase (1982), Arch. Biochem. Biophys., 216, 170-177.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.35 NH4+ Km: 3.7 mM. Monovalent cation required, activation in the order of decreasing efficiency: K+, Rb+, NH4+ Ovis aries

General Stability

EC Number General Stability Organism
2.7.1.35 impure enzyme is stabilized by Zn2+ Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.35 Mg2+ excess free divalent cations inhibit the enzyme Ovis aries
2.7.1.35 Mn2+ excess free divalent cations inhibit the enzyme Ovis aries
2.7.1.35 Zn2+ excess free divalent cations inhibit the enzyme Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.35 additional information
-
 additional information effect of KCl on Km-values Ovis aries
2.7.1.35 0.06
-
 pyridoxine pH 5.8, 37°C, with MnATP2- as the second substrate Ovis aries
2.7.1.35 0.06
-
 ATP pH 5.8, 37°C Ovis aries
2.7.1.35 0.06
-
 ATP ATP in form of ZnATP2- Ovis aries
2.7.1.35 0.101
-
 ATP pH 5.8, 37°C Ovis aries
2.7.1.35 0.101
-
 ATP ATP in form of MgATP2- Ovis aries
2.7.1.35 0.194
-
 ATP pH 5.8, 37°C Ovis aries
2.7.1.35 0.194
-
 ATP ATP in form of MnATP2- Ovis aries

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.35 K+ Km: 8.9 mM. Monovalent cation required, activation in the order of decreasing efficiency: K+, Rb+, NH4+ Ovis aries
2.7.1.35 Rb+ Km: 5.3 mM. Monovalent cation required, activation in the order of decreasing efficiency: K+, Rb+, NH4+ Ovis aries
2.7.1.35 Zn2+ the enzyme requires divalent cations for activity. At 0.08 mM the cations activate in order of decreasing efficiency: Mn2+, Zn2+, Mg2+. At 0.4 mM the cations activate in the order of decreasing efficiency: Mn2+, Zn2+, Mg2+ Ovis aries

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.35 60000
-
 gel filtration, equilibrium sedimentation Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.35 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.35
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.35 liver
-
 Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.35 ATP + pyridoxal
-
 Ovis aries ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxine
-
 Ovis aries ADP + pyridoxine 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.35 monomer 1 * 60000, SDS-PAGE Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.35 5.5 6
-
 Ovis aries