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Literature summary extracted from

  • Fisher, M.N.; Robishaw, J.D.; Neely, J.R.
    The properties and regulation of pantothenate kinase from rat heart (1985), J. Biol. Chem., 260, 15745-15751.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.33 highly unstable upon purification, e.g. successive chromatography Rattus norvegicus
2.7.1.33 thiol reducing agents stabilize enzyme in solution Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 acetyl-CoA
-
 Rattus norvegicus
2.7.1.33 acyl-CoA-esters
-
 Rattus norvegicus
2.7.1.33 ATP free form Rattus norvegicus
2.7.1.33 citrate
-
 Rattus norvegicus
2.7.1.33 CoA not reversible by D-carnitine, acetyl-L-carnitine or other carnitine analogs; reversible by L-carnitine Rattus norvegicus
2.7.1.33 dephospho-CoA
-
 Rattus norvegicus
2.7.1.33 diphosphate
-
 Rattus norvegicus
2.7.1.33 additional information not inhibitory: Mg2+, NADP+; not inhibitory: NAD+ Rattus norvegicus
2.7.1.33 palmitoyl-CoA
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.018
-
 pantothenate pH 7.0, 37°C Rattus norvegicus
2.7.1.33 0.6
-
 ATP pH 7.0, 37°C Rattus norvegicus
2.7.1.33 0.6
-
 ATP ATP in form of MgATP2- Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.33 Mg2+ Km-value: 0.6 mM Rattus norvegicus
2.7.1.33 Mg2+ requirement, active substrate: MgATP-complex Rattus norvegicus
2.7.1.33 additional information nonspecific activation by anions in triethanolamine buffer, pH 7, mostly dicarboxylic acids, not citrate or diphosphate Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + D-pantothenate Rattus norvegicus
-
 ADP + D-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + D-pantothenate Rattus norvegicus Sprague-Dawley
-
 ADP + D-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Rattus norvegicus
-
 Sprague-Dawley
-
2.7.1.33 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.33 heart ventricular muscle Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.33 0.00171
-
 pH 7.0, 37°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.33 -65°C, 3 mg partially purified protein/ml, at least 4 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Rattus norvegicus ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Rattus norvegicus Sprague-Dawley ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + D-pantothenate
-
 Rattus norvegicus ADP + D-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + D-pantothenate
-
 Rattus norvegicus Sprague-Dawley ADP + D-4'-phosphopantothenate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.33 6 9 broad, highest activity in 50 mM phosphate buffer Rattus norvegicus