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Literature summary extracted from

  • Abiko, Y.; Ashida, S.I.; Shimizu, M.
    Purification and properties of D-pantothenate kinase from rat liver (1972), Biochim. Biophys. Acta, 268, 364-372.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.33 ATP and/or sucrose stabilize during purification Rattus norvegicus
2.7.1.33 considerably unstable independent of the degree of purity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 2'-Ketopantetheine
-
 Rattus norvegicus
2.7.1.33 CoA strong Rattus norvegicus
2.7.1.33 dephospho-CoA
-
 Rattus norvegicus
2.7.1.33 L-Pantothenate
-
 Rattus norvegicus
2.7.1.33 pantetheine 4'-phosphate strong Rattus norvegicus
2.7.1.33 pantothenoylcysteine 4'-phosphate strong Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.011
-
 pantothenate pH 6.1, 37°C Rattus norvegicus
2.7.1.33 1
-
 ATP pH 6.1, 37°C Rattus norvegicus
2.7.1.33 1
-
 ATP ATP in form of MgATP2- Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.33 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.33 Mg2+ requirement, active substrate: MgATP-complex Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + D-pantothenate Rattus norvegicus first and rate-controlling reaction of CoA-biosynthesis ADP + D-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Rattus norvegicus
-
 male Wistar
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.33 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.33 0.0041
-
 pH 6.1, 37°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.33 -20°C, above 3 mg protein/ml, more than 1 week Rattus norvegicus
2.7.1.33 4°C, above 3 mg protein/ml, 4 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Rattus norvegicus ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + D-pantothenate D-configuration of 2'-hydroxyl group in pantothenate molecule is essential for functional interaction with enzyme Rattus norvegicus ADP + D-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + D-pantothenate first and rate-controlling reaction of CoA-biosynthesis Rattus norvegicus ADP + D-4'-phosphopantothenate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.33 5.9 6.3
-
 Rattus norvegicus