EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.32 | ADP | 50% inhibition at 0.32 mM, in a positive cooperative manner | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.32 | 0.09 | - |
ATP | pH 9.5, 30°C | Saccharomyces cerevisiae | |
2.7.1.32 | 0.27 | - |
choline | pH 9.5, 30°C | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.32 | cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.32 | Mg2+ | required | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 66300 | - |
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as tetramer and octamer whose amounts increase in the presence of ATP | Saccharomyces cerevisiae |
2.7.1.32 | 66300 | - |
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as dimer and octamer, amount of tetramer and octamer increases in the presence of ATP | Saccharomyces cerevisiae |
2.7.1.32 | 73000 | - |
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as tetramer and octamer whose amounts increase in the presence of ATP | Saccharomyces cerevisiae |
2.7.1.32 | 73000 | - |
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as dimer and octamer, amount of tetramer and octamer increases in the presence of ATP | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.32 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.32 | recombinant enzyme, from Sf-9 insect cells | Saccharomyces cerevisiae |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.32 | ATP + choline = ADP + phosphocholine | sequential bi bi reaction | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.32 | ATP + choline | - |
Saccharomyces cerevisiae | ADP + O-phosphocholine | - |
r | |
2.7.1.32 | ATP + ethanolamine | poor substrate compared to choline | Saccharomyces cerevisiae | ADP + ethanolamine phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.32 | dimer | 2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as tetramer and octamer whose amounts increase in the presence of ATP | Saccharomyces cerevisiae |
2.7.1.32 | tetramer | 2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as dimer and octamer, amount of tetramer and octamer increases in the presence of ATP | Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 30 | - |
- |
Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.32 | 2.55 | - |
choline | per subunit | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 9.5 | - |
- |
Saccharomyces cerevisiae |