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Literature summary extracted from

  • Kim, K.H.; Voelker, D.R.; Flocco, M.T.; Carman, G.M.
    Expression, purification, and characterization of choline kinase, product of the CKI gene from Saccharomyces cerevisiae (1998), J. Biol. Chem., 273, 6844-6852.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 ADP 50% inhibition at 0.32 mM, in a positive cooperative manner Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.32 0.09
-
ATP pH 9.5, 30°C Saccharomyces cerevisiae
2.7.1.32 0.27
-
choline pH 9.5, 30°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.32 cytosol
-
Saccharomyces cerevisiae 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.32 Mg2+ required Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.32 66300
-
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as tetramer and octamer whose amounts increase in the presence of ATP Saccharomyces cerevisiae
2.7.1.32 66300
-
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as dimer and octamer, amount of tetramer and octamer increases in the presence of ATP Saccharomyces cerevisiae
2.7.1.32 73000
-
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as tetramer and octamer whose amounts increase in the presence of ATP Saccharomyces cerevisiae
2.7.1.32 73000
-
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as dimer and octamer, amount of tetramer and octamer increases in the presence of ATP Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32 recombinant enzyme, from Sf-9 insect cells Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.32 ATP + choline = ADP + phosphocholine sequential bi bi reaction Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
Saccharomyces cerevisiae ADP + O-phosphocholine
-
r
2.7.1.32 ATP + ethanolamine poor substrate compared to choline Saccharomyces cerevisiae ADP + ethanolamine phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.32 dimer 2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as tetramer and octamer whose amounts increase in the presence of ATP Saccharomyces cerevisiae
2.7.1.32 tetramer 2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as dimer and octamer, amount of tetramer and octamer increases in the presence of ATP Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.32 30
-
-
Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.32 2.55
-
choline per subunit Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.32 9.5
-
-
Saccharomyces cerevisiae