EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.82 | choline | ethanolamine kinase I not inhibited, ethanolamine kinase II strongly inhibited | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.32 | 0.03 | - |
choline | - |
Rattus norvegicus | |
2.7.1.32 | 3.7 | - |
N-Monomethylethanolamine | cosubstrate ATP | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.82 | Ca2+ | can substitute for Mg2+ | Rattus norvegicus | |
2.7.1.82 | Mg2+ | ethanolamine kinase I: uses MgATP2- as substrate but also has an additional requirement for Mg2+ | Rattus norvegicus | |
2.7.1.82 | Mg2+ | maximal activation at 16 mM MgATP2-, inhibition above | Rattus norvegicus | |
2.7.1.82 | Mg2+ | Km MgATP2-: 14 mM | Rattus norvegicus | |
2.7.1.82 | Mg2+ | ethanolamine kinase II: requires MgATP2- as substrate, maximal activation with 10 mM of Mg2+ and ATP | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.82 | 36000 | - |
ethanolamine kinase I, gel filtration | Rattus norvegicus |
2.7.1.82 | 160000 | - |
ethanolamine kinase II, gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.32 | Rattus norvegicus | - |
- |
- |
2.7.1.82 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.82 | ethanolamine kinase I and II | Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.32 | ATP + choline = ADP + phosphocholine | ethanolamine kinase II and choline kinase do not use a common active site | Rattus norvegicus | |
2.7.1.82 | ATP + ethanolamine = ADP + O-phosphoethanolamine | ethanolamine kinase II and choline kinase do not use a common active site | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.82 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 0.169 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.82 | -20°C, 0.02 M phosphate buffer, pH 7.0, 2.9 mM DTT, protein concentration 1.0 mg/ml, ethanolamine kinase I, 2 days, 40% loss of activity | Rattus norvegicus |
2.7.1.82 | 4°C, 1 week, ethanolamine kinase I, 50% loss of activity | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.32 | ATP + choline | - |
Rattus norvegicus | ADP + O-phosphocholine | - |
? | |
2.7.1.82 | ATP + ethanolamine | - |
Rattus norvegicus | ADP + O-phosphoethanolamine | - |
? | |
2.7.1.82 | choline + ATP | ethanolamine kinase II, not ethanolamine kinase I | Rattus norvegicus | ADP + O-phosphocholine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.82 | More | cf. EC 2.7.1.32 | Rattus norvegicus |
2.7.1.82 | More | ethanolamine kinase II and choline kinase do not use a common active site | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 8 | - |
- |
Rattus norvegicus |
2.7.1.82 | 7.5 | 8.5 | ethanolamine kinase I | Rattus norvegicus |
2.7.1.82 | 8 | 9.5 | ethanolamine kinase II | Rattus norvegicus |