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Literature summary extracted from

  • Yoshida, T.; Fukuta, K.; Mitsunaga, T.; Yamada, H.; Izumi, Y.
    Purification and characterization of glycerate kinase from a serine-producing methylotroph, Hyphomicrobium methylovorum GM2 (1992), Eur. J. Biochem., 210, 849-854.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.165 2,3-diphospho-D-glycerate
-
Hyphomicrobium methylovorum
2.7.1.165 3-phosphoglycerate
-
Hyphomicrobium methylovorum
2.7.1.165 AgNO3
-
Hyphomicrobium methylovorum
2.7.1.165 CuCl2
-
Hyphomicrobium methylovorum
2.7.1.165 HgCl2
-
Hyphomicrobium methylovorum
2.7.1.165 Hydroxypyruvate
-
Hyphomicrobium methylovorum
2.7.1.165 L-Glycerate
-
Hyphomicrobium methylovorum
2.7.1.165 p-chloromercuribenzoate
-
Hyphomicrobium methylovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.165 0.13
-
ATP cosubstrate D-glycerate, at pH 7.0, 30°C Hyphomicrobium methylovorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.165 Co2+ 10 mM, 75% of the activity with Mg2+ Hyphomicrobium methylovorum
2.7.1.165 K+ 50 mM, high stimulation Hyphomicrobium methylovorum
2.7.1.165 Mg2+ required Hyphomicrobium methylovorum
2.7.1.165 Mg2+ 10 mM, activates Hyphomicrobium methylovorum
2.7.1.165 Mn2+ 10 mM, 72% of the activity with 10 mM Mg2+ Hyphomicrobium methylovorum
2.7.1.165 NH4+ 50 mM, high stimulation Hyphomicrobium methylovorum
2.7.1.165 Ni2+ stimulates, 10 mM, 29% of the activity with Mg2+ Hyphomicrobium methylovorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.165 41000 48000 gel filtration Hyphomicrobium methylovorum
2.7.1.165 52000
-
1 * 52000, SDS-PAGE Hyphomicrobium methylovorum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.165 Hyphomicrobium methylovorum
-
GM2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.165
-
Hyphomicrobium methylovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.165 181
-
pH 7.0, 30°C Hyphomicrobium methylovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.165 ATP + (R)-glycerate activity towards L-glycerate is 13% of that towards D-glycerate Hyphomicrobium methylovorum ADP + 3-phospho-(R)-glycerate 2-phosphoglycerate ir
2.7.1.165 CTP + (R)-glycerate at 59% of the activity with ATP Hyphomicrobium methylovorum CDP + 3-phospho-(R)-glycerate
-
?
2.7.1.165 GTP + (R)-glycerate at 59% of the activity with ATP Hyphomicrobium methylovorum GDP + 3-phospho-(R)-glycerate
-
?
2.7.1.165 UTP + (R)-glycerate at 64% of the activity with ATP Hyphomicrobium methylovorum UDP + 3-phospho-(R)-glycerate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.165 monomer 1 * 52000, SDS-PAGE Hyphomicrobium methylovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.165 50
-
-
Hyphomicrobium methylovorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.165 35
-
30 min, stable Hyphomicrobium methylovorum
2.7.1.165 40
-
30 min, 14.3% loss of activity Hyphomicrobium methylovorum
2.7.1.165 50
-
30 min, 52.4% loss of activity Hyphomicrobium methylovorum
2.7.1.165 60
-
30 min, 87.3% loss of activity Hyphomicrobium methylovorum
2.7.1.165 70
-
30 min, 100% loss of activity Hyphomicrobium methylovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.165 8
-
-
Hyphomicrobium methylovorum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.165 6 10 30°C, 30 min, stable Hyphomicrobium methylovorum