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Literature summary extracted from

  • Huang, H.S.; Yoshida, T.; Meng, Y.; Kabashima, T.; Ito, K.; Nishiya, Y.; Kawamura, Y.; Yoshimoto, T.
    Purification and characterization of thermostable glycerol kinase from Thermus flavus (1997), J. Ferment. Bioeng., 83, 328-332.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.30 HgCl2
-
 Thermus thermophilus
2.7.1.30 Mn2+
-
 Thermus thermophilus
2.7.1.30 p-chloromercuribenzoate
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.30 0.038
-
 ATP
-
 Thermus thermophilus
2.7.1.30 0.162
-
 glycerol
-
 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 58000
-
 4 * 58000, SDS-PAGE Thermus thermophilus
2.7.1.30 220000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.30
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ATP + glycerol
-
 Thermus thermophilus ADP + sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.30 tetramer 4 * 58000, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.30 50 70
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.30 68
-
 30 min, pH 7.5, 50% activity Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.30 9 9.5
-
 Thermus thermophilus