Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Comer, M.J.; Bruton, C.J.; Atkinson, T.
    Purification and properties of glycerokinase from Bacillus stearothermophilus (1979), J. Appl. Biochem., 1, 259-270.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.30 fructose 1,6-diphosphate
-
 Escherichia coli
2.7.1.30 fructose 1,6-diphosphate
-
 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.30 additional information
-
 additional information
-
 Escherichia coli
2.7.1.30 additional information
-
 additional information
-
 Geobacillus stearothermophilus
2.7.1.30 additional information
-
 additional information
-
 Candida mycoderma
2.7.1.30 0.15
-
 glyceric acid
-
 Escherichia coli
2.7.1.30 0.15
-
 glyceric acid
-
 Geobacillus stearothermophilus
2.7.1.30 0.15
-
 glyceric acid
-
 Candida mycoderma
2.7.1.30 4.9
-
 monothioglycerol
-
 Escherichia coli
2.7.1.30 4.9
-
 monothioglycerol
-
 Candida mycoderma
2.7.1.30 4.9
-
 monothioglycerol 30°C Geobacillus stearothermophilus
2.7.1.30 42
-
 L-glyceraldehyde
-
 Escherichia coli
2.7.1.30 42
-
 L-glyceraldehyde
-
 Candida mycoderma
2.7.1.30 42
-
 L-glyceraldehyde 30°C Geobacillus stearothermophilus
2.7.1.30 100
-
 dihydroxyacetone
-
 Escherichia coli
2.7.1.30 100
-
 dihydroxyacetone
-
 Candida mycoderma
2.7.1.30 100
-
 dihydroxyacetone 30°C Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 58000
-
 4 * 58000, SDS-PAGE Geobacillus stearothermophilus
2.7.1.30 230000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Candida mycoderma
-
-
-
2.7.1.30 Escherichia coli
-
-
-
2.7.1.30 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.30
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.30 116
-
 30°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ATP + dihydroxyacetone
-
 Escherichia coli ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.30 ATP + dihydroxyacetone
-
 Geobacillus stearothermophilus ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.30 ATP + dihydroxyacetone
-
 Candida mycoderma ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.30 ATP + glyceric acid
-
 Geobacillus stearothermophilus ADP + ?
-
 ?
2.7.1.30 ATP + glyceric acid
-
 Escherichia coli ?
-
 ?
2.7.1.30 ATP + glyceric acid
-
 Candida mycoderma ?
-
 ?
2.7.1.30 ATP + glycerol
-
 Escherichia coli ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol
-
 Geobacillus stearothermophilus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol
-
 Candida mycoderma ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + L-glyceraldehyde
-
 Escherichia coli ADP + L-glyceraldehyde 3-phosphate
-
 ?
2.7.1.30 ATP + L-glyceraldehyde
-
 Geobacillus stearothermophilus ADP + L-glyceraldehyde 3-phosphate
-
 ?
2.7.1.30 ATP + L-glyceraldehyde
-
 Candida mycoderma ADP + L-glyceraldehyde 3-phosphate
-
 ?
2.7.1.30 ATP + mercaptopropanediol
-
 Escherichia coli 1-mercaptopropanediol 1-phosphate + ADP
-
 ?
2.7.1.30 ATP + mercaptopropanediol
-
 Candida mycoderma 1-mercaptopropanediol 1-phosphate + ADP
-
 ?
2.7.1.30 ATP + monothioglycerol
-
 Geobacillus stearothermophilus ADP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.30 tetramer
-
 Escherichia coli
2.7.1.30 tetramer 4 * 58000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.30 30
-
 assay at Geobacillus stearothermophilus
2.7.1.30 65
-
-
 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.30 30 80 30°C: about 50% of maximum activity, 80°C: about 65% of maximum activity Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.30 20
-
 no loss of activity detectable Geobacillus stearothermophilus
2.7.1.30 20
-
 t1/2 8.6 days Escherichia coli
2.7.1.30 20
-
 t1/2: 4.1 days Candida mycoderma
2.7.1.30 60
-
 t1/2: 310 min Geobacillus stearothermophilus
2.7.1.30 60
-
 t1/2: 0.72 min Candida mycoderma
2.7.1.30 60
-
 t1/2: 4.5 min Escherichia coli
2.7.1.30 70
-
-
 Escherichia coli
2.7.1.30 70
-
-
 Candida mycoderma
2.7.1.30 70
-
 t1/2: 3 min Geobacillus stearothermophilus
2.7.1.30 80
-
 t1/2: 0.53 min Geobacillus stearothermophilus