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Literature summary extracted from

  • Nilsson, A.; Thomson, K.S.; Adler, L.
    Purification and characterization of glycerol kinase in the salt-tolerant yeast Debaryomyces hansenii (1989), Biochim. Biophys. Acta, 991, 296-302.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.30 (NH4)2SO4
-
 Debaryomyces hansenii
2.7.1.30 ADP product inhibition Debaryomyces hansenii
2.7.1.30 AMP
-
 Debaryomyces hansenii
2.7.1.30 fructose 1,6-diphosphate weak inhibition Debaryomyces hansenii
2.7.1.30 Na2SO4
-
 Debaryomyces hansenii
2.7.1.30 NaCl
-
 Debaryomyces hansenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.30 additional information
-
 additional information
-
 Debaryomyces hansenii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.30 KCl stimulates Debaryomyces hansenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 236000
-
 gel filtration Debaryomyces hansenii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Debaryomyces hansenii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.30
-
 Debaryomyces hansenii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.30 177
-
 pH 7.5, 25°C Debaryomyces hansenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ATP + glycerol only glycerol active as phosphoryl group acceptor Debaryomyces hansenii ADP + sn-glycerol 3-phosphate
-
 ir
2.7.1.30 CTP + glycerol 14% of the activity with ATP Debaryomyces hansenii CDP + glycerol 3-phosphate
-
 ?
2.7.1.30 UTP + glycerol 7% of the activity with ATP Debaryomyces hansenii UDP + glycerol 3-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.30 7 8
-
 Debaryomyces hansenii