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Literature summary extracted from

  • Hayashi, S.; Lin, E.C.C.
    Purification and properties of glycerol kinase from Escherichia coli (1967), J. Biol. Chem., 242, 1030-1035.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.30
-
 Escherichia coli

General Stability

EC Number General Stability Organism
2.7.1.30 glycerol, 0.01 M, EDTA, 0.001 M and 0.001 M 2-mercaptoethanol prevent inactivation during purification Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.30 fructose 1,6-diphosphate
-
 Escherichia coli
2.7.1.30 L-alpha-glycerophosphate no inhibition up to 3 mM in presence of 0.1 M glycerol Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.30 additional information
-
 additional information
-
 Escherichia coli
2.7.1.30 0.5
-
 dihydroxyacetone 25°C, plus D-glyceraldehyde Escherichia coli
2.7.1.30 3
-
 L-glyceraldehyde 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.30 Mg2+ required Escherichia coli
2.7.1.30 Mn2+ can substitute for Mg2+ Escherichia coli
2.7.1.30 Mn2+ less active at equal molar concentration than Mg2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 280000 300000 sedimentation equilibrium light scattering method Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.30
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.30 100
-
 25°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ATP + D-glyceraldehyde
-
 Escherichia coli ADP + D-glyceraldehyde 3-phosphate
-
 ?
2.7.1.30 ATP + dihydroxyacetone
-
 Escherichia coli ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.30 ATP + glycerol high specificity for ATP, no other ribonucleoside triphosphate utilized Escherichia coli ADP + sn-glycerol 3-phosphate i.e. L-alpha-glycerophosphate ?
2.7.1.30 ATP + L-glyceraldehyde
-
 Escherichia coli ADP + L-glyceraldehyde 3-phosphate
-
 ?
2.7.1.30 additional information D-glyceraldehyde promotes conversion of ATP to ADP + phosphate Escherichia coli ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.30 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.30 9.8
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.30 6.8 10.5 pH 6.8: about 30% of maximum activity, pH 10.5: about 80% of maximum activity Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.30 6 7 0°C, half-life: 24 h Escherichia coli