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Literature summary extracted from

  • Krakow, J.L.; Wang, C.C.
    Purification and characterization of glycerol kinase from Trypanosoma brucei (1990), Mol. Biochem. Parasitol., 43, 17-25.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.30 ADP weak, competitive to ATP, uncompetitive to glycerol Trypanosoma brucei
2.7.1.30 glycerol 3-phosphate up to 10 mM, no product inhibition of the forward reaction Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.30 0.17
-
 glycerol pH 9.0 Trypanosoma brucei
2.7.1.30 0.19
-
 ATP pH 7.4 Trypanosoma brucei
2.7.1.30 0.26
-
 glycerol pH 7.4, ATP, pH 9.0 Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.30 glycosome
-
 Trypanosoma brucei 20015
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 53000
-
-
 Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.30
-
 Trypanosoma brucei

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.30 ATP + glycerol = ADP + sn-glycerol 3-phosphate random bi bi mechanism Trypanosoma brucei
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ATP + glycerol
-
 Trypanosoma brucei ADP + sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.30 monomer 1 * 53000 Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.30 7 9.5
-
 Trypanosoma brucei