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Literature summary extracted from

  • Bergmeyer, H.U.; Holz, G.; Kauder, E.M.; Möllering, H.; Wieland, O.
    Kristallisierte Glycerokinase aus Candida mycoderma (1961), Biochem. Z., 333, 471-480.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.30
-
 Candida mycoderma

General Stability

EC Number General Stability Organism
2.7.1.30 dialysis, against 0.01 M phosphate buffer, at 2°C, pH 6.7, 50% loss of activity without glycerol, stable in presence of 0.01 M glycerol Candida mycoderma

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.30 iodoacetamide
-
 Candida mycoderma
2.7.1.30 p-chloromercuribenzoate
-
 Candida mycoderma
2.7.1.30 Salyrganic acid
-
 Candida mycoderma

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.30 0.06
-
 glycerol 25°C Candida mycoderma
2.7.1.30 0.09
-
 ATP 25°C Candida mycoderma

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 251000
-
 diffusion and sedimentation data Candida mycoderma

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Candida mycoderma
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.30
-
 Candida mycoderma

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.30 mycelium
-
 Candida mycoderma
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.30 101
-
 25°C Candida mycoderma

Storage Stability

EC Number Storage Stability Organism
2.7.1.30 4°C, crystallized suspension in 2.2 M ammonium sulfate, stable for several months Candida mycoderma

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ATP + dihydroxyacetone
-
 Candida mycoderma ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.30 ATP + glycerol
-
 Candida mycoderma ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 GTP + glycerol
-
 Candida mycoderma GDP + glycerol 3-phosphate
-
 ?
2.7.1.30 ITP + glycerol
-
 Candida mycoderma IDP + glycerol 3-phosphate
-
 ?
2.7.1.30 UTP + glycerol
-
 Candida mycoderma UDP + glycerol 3-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.30 25
-
 assay at Candida mycoderma

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.30 1670
-
 glycerol
-
 Candida mycoderma

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.30 6.7
-
 highest stability Candida mycoderma