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Literature summary extracted from

  • Gutknecht, R.; Beutler, R.; Garcia-Alles, L.F.; Baumann, U.; Erni, B.
    The dihydroxyacetone kinase of Escherichia coli utilizes a phosphoprotein instead of ATP as phosphoryl donor (2001), EMBO J., 20, 2480-2486.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.29 overexpression in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.29 H169A completely inactive Escherichia coli
2.7.1.29 H439A completely inactive Escherichia coli
2.7.1.29 H9A completely inactive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.29 0.045
-
 dihydroxyacetone pH 7.5, 30°C, DhaK-DhaL-DhaM complex Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.29 52000
-
 2 * 52000 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.29 Escherichia coli P37349 phosphoryl donor DhaM
-
2.7.1.29 Escherichia coli P76014 DhaL subunit
-
2.7.1.29 Escherichia coli P76015 DhaK subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.29 DEAE-cellulose, Superdex 75 Escherichia coli
2.7.1.29 HighQ anion exchange, Superdex 200 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.29 phosphorylated DhaM domain of dihydroxyacetone kinase + dihydroxyacetone enzyme complex uses the PEP:sugar phosphotransferase protein DhaM instead of ATP as phosphoryl donor Escherichia coli dephospho-DhaM + dihydroxyacetone phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.29 dimer 2 * 52000 Escherichia coli
2.7.1.29 More approx. 18000 Da, monomeric state is suggested Escherichia coli
2.7.1.29 More approx. 35000 Da, monomeric state is suggested Escherichia coli
2.7.1.29 More enzyme comlex is present in an approx. 1/1/1 ratio of DhaK, DhaL and DhaM Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.29 2.8
-
 dihydroxyacetone pH 7.5, 30°C, DhaK-DhaL-DhaM complex Escherichia coli