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Literature summary extracted from

  • Bystrykh, L.V.; de Koning, W.; Harder, W.
    Triokinase from Candida boidinii KD1 (1990), Methods Enzymol., 188, 445-451.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.29 glycerol, most effective stabilizer, EDTA and MgCl2 stabilize to a lesser extent, inactivated during ammonium sulfate precipitation [Candida] boidinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.29 Be2+
-
 [Candida] boidinii
2.7.1.29 Ca2+
-
 [Candida] boidinii
2.7.1.29 Co2+
-
 [Candida] boidinii
2.7.1.29 Cu2+
-
 [Candida] boidinii
2.7.1.29 Mn2+
-
 [Candida] boidinii
2.7.1.29 additional information both components of the MgATP complex inhibit the kinase at excess concentration [Candida] boidinii
2.7.1.29 Zn2+
-
 [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.29 0.35
-
 ATP pH 8.0, 30-37°C [Candida] boidinii
2.7.1.29 0.35
-
 ATP ATP in form of MgATP2- [Candida] boidinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.29 Mg2+ required for activity, no other cation can replace Mg2+ [Candida] boidinii
2.7.1.29 additional information no tightly bound metal ions [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.29 71000
-
 2 * 71000, SDS-PAGE [Candida] boidinii
2.7.1.29 139000
-
-
 [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.29 ATP + dihydroxyacetone [Candida] boidinii second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.29 ATP + dihydroxyacetone [Candida] boidinii KD1 second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol ADP + dihydroxyacetone phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.29 [Candida] boidinii
-
-
-
2.7.1.29 [Candida] boidinii KD1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.29 polyethylene glycol 6000, polyethyleneimine, DEAE-Sepharose, Sepharose CL-6B [Candida] boidinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.29 20.33
-
-
 [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.29 ATP + dihydroxyacetone
-
 [Candida] boidinii ADP + dihydroxyacetone phosphate
-
 ir
2.7.1.29 ATP + dihydroxyacetone second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol [Candida] boidinii ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.29 ATP + dihydroxyacetone
-
 [Candida] boidinii KD1 ADP + dihydroxyacetone phosphate
-
 ir
2.7.1.29 ATP + dihydroxyacetone second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol [Candida] boidinii KD1 ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.29 ATP + DL-glyceraldehyde 25% of the activity with dihydroxyacetone [Candida] boidinii ADP + glyceraldehyde 3-phosphate
-
 ?
2.7.1.29 ATP + glycerone
-
 [Candida] boidinii ADP + glycerone phosphate
-
 ?
2.7.1.29 CTP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii CDP + dihydroxyacetone phosphate
-
 ?
2.7.1.29 CTP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii KD1 CDP + dihydroxyacetone phosphate
-
 ?
2.7.1.29 GTP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii GDP + dihydroxyacetone phosphate
-
 ?
2.7.1.29 GTP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii KD1 GDP + dihydroxyacetone phosphate
-
 ?
2.7.1.29 ITP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii IDP + dihydroxyacetone phosphate
-
 ?
2.7.1.29 ITP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii KD1 IDP + dihydroxyacetone phosphate
-
 ?
2.7.1.29 UTP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii UDP + dihydroxyacetone phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.29 dimer 2 * 71000, SDS-PAGE [Candida] boidinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.29 30
-
 assay at [Candida] boidinii
2.7.1.29 37
-
 assay at [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.29 7.8 8.2
-
 [Candida] boidinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.29 6.7 8.7 optimal stability [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.29 additional information no tightly bound cofactor [Candida] boidinii