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Literature summary extracted from

  • Lillo, A.M.; Tetzlaff, C.N.; Sangari, F.J.; Cane, D.E.
    Functional expression and characterization of eryA, the erythritol kinase of Brucella abortus, and enzymatic synthesis of L-erythritol-4-phosphate (2003), Bioorg. Med. Chem. Lett., 13, 737-739.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.27 expression in Escherichia coli Brucella abortus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.27 0.26
-
 meso-erythritol pH 8.0, 25°C, recombinant erythritol kinase Brucella abortus
2.7.1.27 9.8
-
 ATP pH 8.0, 25°C, recombinant erythritol kinase Brucella abortus
2.7.1.215 0.26
-
 meso-erythritol pH 8.0, 22°C Brucella abortus
2.7.1.215 9.8
-
 ATP pH 8.0, 22°C Brucella abortus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.27 Brucella abortus
-
-
-
2.7.1.215 Brucella abortus Q2YIQ1
-
-
2.7.1.215 Brucella abortus 2308 Q2YIQ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.27 recombinant enzyme, nickel affinity chromatography Brucella abortus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.27 ATP + erythritol
-
 Brucella abortus ADP + D-erythritol 1-phosphate
-
 ?
2.7.1.27 ATP + meso-erythritol
-
 Brucella abortus ADP + meso-erythritol 1-phosphate
-
 ?
2.7.1.215 ATP + meso-erythritol
-
 Brucella abortus ADP + D-erythritol 1-phosphate
-
 ?
2.7.1.215 ATP + meso-erythritol
-
 Brucella abortus 2308 ADP + D-erythritol 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.215 eryA
-
 Brucella abortus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.27 230
-
 ATP pH 8.0, 25°C, recombinant erythritol kinase Brucella abortus
2.7.1.27 230
-
 meso-erythritol pH 8.0, 25°C, recombinant erythritol kinase Brucella abortus
2.7.1.215 230
-
 ATP pH 8.0, 22°C Brucella abortus
2.7.1.215 230
-
 meso-erythritol pH 8.0, 22°C Brucella abortus