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Literature summary extracted from

  • Mack, M.; van Loon, A.P.; Hohmann, H.P.
    Regulation of riboflavin biosynthesis in Bacillus subtilis is affected by the activity of the flavokinase/flavin adenine dinucleotide synthetase encoded by ribC (1998), J. Bacteriol., 180, 950-955.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.26 expression of ribC gene in Escherichia coli Bacillus subtilis
2.7.7.2 ribC encodes a bifunctional flavokinase/FAD-synthetase, cloned and overexpressed in Escherichia coli BL21 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.26 34200
-
 gel filtration Bacillus subtilis
2.7.1.26 36000
-
 1 * 36000, SDS-PAGE Bacillus subtilis
2.7.7.2 34180
-
 wild-type, gel filtration Bacillus subtilis
2.7.7.2 34200
-
 gel filtration, nondenaturing conditions Bacillus subtilis
2.7.7.2 34230
-
 mutant RibC, gel filtration Bacillus subtilis
2.7.7.2 35670
-
 mass spectroscopy Bacillus subtilis
2.7.7.2 35670
-
 deduced from RibC open reading frame Bacillus subtilis
2.7.7.2 36000
-
 wild-type, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.26 ATP + riboflavin Bacillus subtilis ribC is essential for growth of Bacillus subtilis. RibC is not directly involved in the riboflavin regulatory system ADP + FMN
-
 ?
2.7.1.26 ATP + riboflavin Bacillus subtilis 168 ribC is essential for growth of Bacillus subtilis. RibC is not directly involved in the riboflavin regulatory system ADP + FMN
-
 ?
2.7.7.2 ATP + FMN Bacillus subtilis essential for flavin metabolism diphosphate + FAD
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Bacillus subtilis P54575 RibC from wild-type strain 1012 and RibC820 from riboflavin-overproducing mitant strain RB52.ribC wild-type gene product has both flavokinase and flavin adenine dinucleotide synthetase activity
-
2.7.7.2 Bacillus subtilis P54575 1012
-
2.7.7.2 Bacillus subtilis 168 P54575 1012
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.26 recombinant enzyme Bacillus subtilis
2.7.7.2 wild-type and recombinant enzyme Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.2 0.58
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.7.7.2 -20°C, stable for at least 2 weeks Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ATP + riboflavin
-
 Bacillus subtilis ADP + FMN
-
 ?
2.7.1.26 ATP + riboflavin ribC is essential for growth of Bacillus subtilis. RibC is not directly involved in the riboflavin regulatory system Bacillus subtilis ADP + FMN
-
 ?
2.7.1.26 ATP + riboflavin
-
 Bacillus subtilis 168 ADP + FMN
-
 ?
2.7.1.26 ATP + riboflavin ribC is essential for growth of Bacillus subtilis. RibC is not directly involved in the riboflavin regulatory system Bacillus subtilis 168 ADP + FMN
-
 ?
2.7.1.26 additional information ribC wild-type gene product has both flavokinase and flavin adenine dinucleotide synthetase activity Bacillus subtilis ?
-
 ?
2.7.1.26 additional information ribC wild-type gene product has both flavokinase and flavin adenine dinucleotide synthetase activity Bacillus subtilis 168 ?
-
 ?
2.7.7.2 ATP + FMN
-
 Bacillus subtilis diphosphate + FAD
-
 r
2.7.7.2 ATP + FMN essential for flavin metabolism Bacillus subtilis diphosphate + FAD
-
 r
2.7.7.2 additional information bifunctional FAD synthetase which shows FMN adenylyltransferase and flavokinase activities, producing FMN ATP:riboflavin 5'-phosphotransferase EC 2.7.1.26 Bacillus subtilis ?
-
 ?
2.7.7.2 additional information highly purified 5'-FMN is not accepted as a substrate Bacillus subtilis ?
-
 ?
2.7.7.2 additional information bifunctional FAD synthetase which shows FMN adenylyltransferase and flavokinase activities, producing FMN ATP:riboflavin 5'-phosphotransferase EC 2.7.1.26 Bacillus subtilis 168 ?
-
 ?
2.7.7.2 additional information highly purified 5'-FMN is not accepted as a substrate Bacillus subtilis 168 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.26 monomer 1 * 36000, SDS-PAGE Bacillus subtilis
2.7.7.2 monomer 1 * 36000, SDS-PAGE Bacillus subtilis