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Literature summary extracted from

  • Rajeswari, S.R.; Jonnalagadda, V.S.; Jonnalagadda, S.
    Purification and characterization of flavokinase from Neurospora crassa (1999), Indian J. Biochem. Biophys., 36, 137-142.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.26 Carbonate 50 mM, activates Neurospora crassa
2.7.1.26 NO3- 50 mM, activates Neurospora crassa
2.7.1.26 phosphate 50 mM, activates Neurospora crassa
2.7.1.26 SO42- 50 mM, activates Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.26 0.000021
-
 ATP pH 8.5, 30°C Neurospora crassa
2.7.1.26 0.000021
-
 ATP ATP in form of MgATP2- Neurospora crassa
2.7.1.26 0.00012
-
 riboflavin pH 8.5, 30°C Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.26 Co2+ divalent metal ions are essential activators, in the order of decreasing efficiency: Mg2+, Co2+, Zn2+, Cu2+, Cd2+, Fe2+ Neurospora crassa
2.7.1.26 Mg2+ most effective essential activator Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.26 35500
-
 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.26 ATP + riboflavin Neurospora crassa key enzyme in flavin biosynthesis ADP + FMN
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Neurospora crassa
-
 cell wall lacking mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.26
-
 Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.26 2.95
-
-
 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ATP + riboflavin
-
 Neurospora crassa ADP + FMN
-
 ?
2.7.1.26 ATP + riboflavin key enzyme in flavin biosynthesis Neurospora crassa ADP + FMN
-
 ?
2.7.1.26 ATP + riboflavin ATP in form of MgATP2- Neurospora crassa ADP + FMN
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.26 monomer 1 * 35500, SDS-PAGE Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.26 37 57
-
 Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.26 0.1
-
 ATP pH 8.5, 30°C Neurospora crassa
2.7.1.26 0.1
-
 riboflavin pH 8.5, 30°C Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.26 8.5 9
-
 Neurospora crassa