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Literature summary extracted from

  • Merrill, A.H.; McCormick, D.B.
    Preparation and properties of immobilized flavokinase (1979), Biotechnol. Bioeng., 21, 1629-1638.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.26 synthesis immobilized enzyme is effective for phosphorylating riboflavin and numerous riboflavin analogs and provides a facile method for preparing exclusively other synthetic methods, the 5'-phosphates Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.1.26 enzyme immobilized by amide linkage to omega aminoalkyl-agarose-beads has a half-life of three weeks at 25°C Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.26 0.01
-
 riboflavin pH 8.0, 37°C Rattus norvegicus
2.7.1.26 0.2
-
 ATP pH 8, 37°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.26 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ATP + 2-thioriboflavin 30% of the activity with riboflavin Rattus norvegicus ADP + 2-thioriboflavin 5'-phosphate
-
 ?
2.7.1.26 ATP + 3-methylriboflavin 5% of the activity with riboflavin Rattus norvegicus ADP + 3-methylriboflavin 5'-phosphate
-
 ?
2.7.1.26 ATP + 5-deazariboflavin as active as riboflavin Rattus norvegicus ADP + 5-deazariboflavin 5'-phosphate
-
 ?
2.7.1.26 ATP + riboflavin
-
 Rattus norvegicus ADP + FMN
-
 ?
2.7.1.26 ATP + roseoflavin 90% of the activity with riboflavin Rattus norvegicus ADP + roseoflavin 5'-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.26 25
-
 enzyme immobilized by amide linkage to omega aminoalkyl-agarose-beads has a half-life of three weeks Rattus norvegicus