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Literature summary extracted from

  • Mayhew, S.G.; Wassink, J.H.
    A continuous fluorometric assay for flavokinase. Properties of flavokinase from Peptostreptococcus elsdenii (1977), Biochim. Biophys. Acta, 482, 341-347.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.26 loss of activity during repeated freezing and thawing Megasphaera elsdenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.26 0.0047
-
 riboflavin pH 7.2, 37°C Megasphaera elsdenii
2.7.1.26 0.01
-
 riboflavin pH 7.2, 37°C Megasphaera elsdenii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Megasphaera elsdenii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.26 0.0083
-
 continuous fluorometric assay Megasphaera elsdenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ADP + riboflavin 22% of the activity with ATP Megasphaera elsdenii AMP + FMN
-
 ?
2.7.1.26 ATP + 5-deazariboflavin
-
 Megasphaera elsdenii ADP + 5-deazariboflavin 5'-phosphate
-
 ?
2.7.1.26 ATP + riboflavin
-
 Megasphaera elsdenii ADP + FMN
-
 ?
2.7.1.26 GTP + riboflavin no activity Megasphaera elsdenii GDP + riboflavin 5'-phosphate
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.26 20 45 the relative activities at pH 7.2 and 20°C, 25°C, 37°C and 45°C are 0.22, 0.46, 1, and 1.35, respectively Megasphaera elsdenii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.26 7
-
-
 Megasphaera elsdenii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.26 5 8.5 enzyme is active between pH 5 and 8.5 Megasphaera elsdenii