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Literature summary extracted from

  • Kasai, S.; Nakano, H.; Maeda, K.; Matsui, K.
    Purification, properties, and function of flavokinase from rat intestinal mucosa (1990), J. Biochem., 107, 298-303.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.26 50% of the activity is lost on freezing, but the stability of the enzyme is not greatly affected by the period of freezing Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.26 13500
-
gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.26 intestinal mucosa
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.26 0.79
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.26 3°C, MOPS buffer, activity decreases to approximately 35, 30 and 13% of the initial level after 24, 48, and 96 h, respectively Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ATP + 2'-deoxyriboflavin 31% of the activity with riboflavin Rattus norvegicus ADP + 2'-deoxyriboflavin 5'-phosphate
-
?
2.7.1.26 ATP + 8-bromo-8-demethylriboflavin 384% of the activity with riboflavin Rattus norvegicus ADP + 8-bromo-8-demethylriboflavin 5-'phosphate
-
?
2.7.1.26 ATP + 8-chloro-8-demethylriboflavin 122.2% of the activity with riboflavin Rattus norvegicus ADP + 8-chloro-8-demethylriboflavin 5'-phosphate
-
?
2.7.1.26 ATP + 8-demethylriboflavin 110% of the activity with riboflavin Rattus norvegicus ADP + 8-demethylriboflavin 5'-phosphate
-
?
2.7.1.26 ATP + 8-dimethylamino-8-demethylriboflavin 110% of the activity with riboflavin Rattus norvegicus ADP + 8-dimethylamino-8-demethylriboflavin 5'-phosphate
-
?
2.7.1.26 ATP + 8-ethoxy-8-demethylriboflavin 210% of the activity with riboflavin Rattus norvegicus ADP + 8-ethoxy-8-demethylriboflavin 5'-phosphate
-
?
2.7.1.26 ATP + 8-fluoro-8-demethylriboflavin 132.2% of the activity with riboflavin Rattus norvegicus ADP + 8-fluoro-8-demethylriboflavin 5'-phosphate
-
?
2.7.1.26 ATP + 8-iodo-8-demethylriboflavin 334.7% of the activity with riboflavin Rattus norvegicus ADP + 8-iodo-8-demethylriboflavin 5'-phosphate
-
?
2.7.1.26 ATP + 8-methoxy-8-demethylriboflavin 114.5% of the activity with riboflavin Rattus norvegicus ADP + 8-methoxy-8-demethylriboflavin 5'-phosphate
-
?
2.7.1.26 ATP + 8-methylamino-8-demethylriboflavin 237.3% of the activity with riboflavin Rattus norvegicus ADP + 8-methylamino-8-demethylriboflavin 5'-phosphate
-
?
2.7.1.26 ATP + riboflavin
-
Rattus norvegicus ADP + FMN
-
?
2.7.1.26 ATP + roseoflavin 187% of the activity with 8-demethylriboflavin Rattus norvegicus ADP + roseoflavin 5'-phosphate
-
?
2.7.1.26 ATP + roseoflavin 81% of the activity with riboflavin Rattus norvegicus ADP + roseoflavin 5'-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.26 monomer 1 * 13500, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.26 60
-
-
Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.26 9
-
enzyme from intestinal mucosa Rattus norvegicus