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Literature summary extracted from

  • Yamada, Y.; Merrill, A.; McCormick, D.N.
    Probable reaction mechanisms of flavokinase and FAD synthetase from rat liver (1990), Arch. Biochem. Biophys., 278, 125-130.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.26 FMN product inhibition Rattus norvegicus
2.7.1.26 ZnADP- product inhibition Rattus norvegicus
2.7.7.2 diphosphate product inhibition Rattus norvegicus
2.7.7.2 FAD strong product inhibitor, 50% inhibition at 0.006 mM, competitive inhibition against ATP, mixed inhibition against FMN Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.26 0.011
-
 riboflavin
-
 Rattus norvegicus
2.7.7.2 0.0091
-
 FMN pH 8.0, 37°C Rattus norvegicus
2.7.7.2 0.071
-
 ATP pH 8.0, 37°C, MgATP Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.2 cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.2 ATP + FMN Rattus norvegicus biosynthesis of FAD is most likely regulated by this coenzyme as a product at the stage of FAD synthetase reaction diphosphate + FAD
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Rattus norvegicus
-
-
-
2.7.7.2 Rattus norvegicus
-
 rat
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.26 ATP + riboflavin = ADP + FMN ordered bi bi mechanism in which riboflavin binds first followed by ATP. ADP is released first followed by FMN Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.26 liver
-
 Rattus norvegicus
-
2.7.7.2 hepatocyte
-
 Rattus norvegicus
-
2.7.7.2 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ATP + riboflavin
-
 Rattus norvegicus ADP + FMN
-
 ?
2.7.7.2 ATP + FMN
-
 Rattus norvegicus diphosphate + FAD
-
 ir
2.7.7.2 ATP + FMN biosynthesis of FAD is most likely regulated by this coenzyme as a product at the stage of FAD synthetase reaction Rattus norvegicus diphosphate + FAD
-
 ir

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.26 0.006
-
 FMN pH 8.0, 37°C, against riboflavin Rattus norvegicus
2.7.1.26 0.023
-
 ZnADP- pH 8.0, 37°C, against ZnATP2- Rattus norvegicus
2.7.1.26 0.12
-
 ZnADP- pH 8.0, 37°C, against riboflavin Rattus norvegicus
2.7.7.2 additional information
-
 additional information Ki value for diphosphate against ATP not determined because Lineweaver-Burk plots of inhibition by diphosphate with varying concentrations of ATP are nonlinear Rattus norvegicus
2.7.7.2 0.00075
-
 FAD pH 8.0, 37°C, against FMN Rattus norvegicus
2.7.7.2 0.0013
-
 FAD pH 8.0, 37°C, against MgATP 2- Rattus norvegicus
2.7.7.2 0.066
-
 diphosphate pH 8.0, 37°C, against FMN Rattus norvegicus