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Literature summary extracted from

  • MacRae, I.J.; Rose, A.B.; Segel, I.H.
    Adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum. site-directed mutagenesis at putative phosphoryl-accepting and ATP P-loop residues (1998), J. Biol. Chem., 273, 28583-28589.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.25 ammonium sulfate high salt activates at high adenosine 5'-phosphosulfate concentrations but inhibits at low adenosine 5'-phosphosulfate concentrations Penicillium chrysogenum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 expression of wild-type and S107A mutant enzyme in Escherichia coli Penicillium chrysogenum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.25 S104A similar properties as wild-type Penicillium chrysogenum
2.7.1.25 S107A similar properties as wild-type enzyme Penicillium chrysogenum
2.7.1.25 S107C similar properties as wild-type, suggesting that S107 is not essential for activity but may be located in the substrate binding pocket Penicillium chrysogenum
2.7.1.25 S97A similar properties as wild-type Penicillium chrysogenum
2.7.1.25 S99A similar properties as wild-type Penicillium chrysogenum
2.7.1.25 T103A similar properties as wild-type Penicillium chrysogenum
2.7.1.25 Y109F similar properties as wild-type, velocity curve is shifted to the far right Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 ammonium sulfate high salt inhibits at low adenosine 5'-phosphosulfate concentrations, but activates at high adenosine 5'-phosphosulfate concentrations Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.25 0.001
-
 adenosine 5'-phosphosulfate pH 8.1, 30°C, wild-type enzyme at 100 mM ammonium sulfate Penicillium chrysogenum
2.7.1.25 0.003
-
 3'-phosphoadenosine 5'-phosphosulfate pH 8.1, 30°C, wild-type enzyme Penicillium chrysogenum
2.7.1.25 0.012
-
 adenosine 5'-phosphosulfate pH 8.1, 30°C, S107A mutant enzyme at 100 mM ammonium sulfate Penicillium chrysogenum
2.7.1.25 0.039
-
 3'-phosphoadenosine 5'-phosphosulfate pH 8.1, 30°C, S107A mutant enzyme Penicillium chrysogenum
2.7.1.25 0.8
-
 ATP pH 8.1, 30°C, wild-type enzyme at 100 mM ammonium sulfate Penicillium chrysogenum
2.7.1.25 0.8
-
 ATP ATP in form of MgATP2- Penicillium chrysogenum
2.7.1.25 2.4
-
 ATP pH 8.1, 30°C, S107A mutant enzyme Penicillium chrysogenum
2.7.1.25 2.4
-
 ATP ATP in form of MgATP2- Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Escherichia coli P23846
-
-
2.7.1.25 Penicillium chrysogenum Q12657
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
2.7.1.25 ATP + adenylyl sulfate
-
 Penicillium chrysogenum ADP + 3'-phosphoadenylyl sulfate
-
 r