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Literature summary extracted from

  • Satishchandran, C.; Hickman, Y.N.; Markham, G.D.
    Characterization of the phosphorylated enzyme intermediate formed in the adenosine 5-phosphosulfate kinase reaction (1992), Biochemistry, 31, 11684-11688.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 structural gene cysC Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 22321
-
x * 22321, deduced from DNA sequence of cysC coding region Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate Escherichia coli ultimate step in biosynthesis of 3'-phosphoadenosine 5'-phosphosulfate, the primary biological sulfuryl donor ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Escherichia coli
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.25 side-chain modification enzyme is phosphorylated upon incubation with ATP, phosphorylation site is identified as Ser109, phosphorylated enzyme is kinetically competent Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25
-
Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.25 ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate mechanism Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate in the absence of adenosine 5'-phosphosulfate ATP phosphorylates the enzyme at a rate equivalent to the overall kinase reaction, phosphorylation site: Ser-109 Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
2.7.1.25 ATP + adenosine 5-phosphosulfate ultimate step in biosynthesis of 3'-phosphoadenosine 5'-phosphosulfate, the primary biological sulfuryl donor Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 ? x * 22321, deduced from DNA sequence of cysC coding region Escherichia coli