Literature summary extracted from
Satishchandran, C.; Hickman, Y.N.; Markham, G.D.
Characterization of the phosphorylated enzyme intermediate formed in the adenosine 5-phosphosulfate kinase reaction (1992), Biochemistry, 31, 11684-11688.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.1.25 |
structural gene cysC |
Escherichia coli |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.1.25 |
22321 |
- |
x * 22321, deduced from DNA sequence of cysC coding region |
Escherichia coli |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.7.1.25 |
ATP + adenosine 5-phosphosulfate |
Escherichia coli |
ultimate step in biosynthesis of 3'-phosphoadenosine 5'-phosphosulfate, the primary biological sulfuryl donor |
ADP + 3'-phosphoadenosine 5'-phosphosulfate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.1.25 |
Escherichia coli |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
2.7.1.25 |
side-chain modification |
enzyme is phosphorylated upon incubation with ATP, phosphorylation site is identified as Ser109, phosphorylated enzyme is kinetically competent |
Escherichia coli |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.1.25 |
- |
Escherichia coli |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.7.1.25 |
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate |
mechanism |
Escherichia coli |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.1.25 |
ATP + adenosine 5-phosphosulfate |
in the absence of adenosine 5'-phosphosulfate ATP phosphorylates the enzyme at a rate equivalent to the overall kinase reaction, phosphorylation site: Ser-109 |
Escherichia coli |
ADP + 3'-phosphoadenosine 5'-phosphosulfate |
i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate |
? |
|
2.7.1.25 |
ATP + adenosine 5-phosphosulfate |
i.e. adenylylsulfate or APS |
Escherichia coli |
ADP + 3'-phosphoadenosine 5'-phosphosulfate |
i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate |
? |
|
2.7.1.25 |
ATP + adenosine 5-phosphosulfate |
ultimate step in biosynthesis of 3'-phosphoadenosine 5'-phosphosulfate, the primary biological sulfuryl donor |
Escherichia coli |
ADP + 3'-phosphoadenosine 5'-phosphosulfate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.1.25 |
? |
x * 22321, deduced from DNA sequence of cysC coding region |
Escherichia coli |