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Literature summary extracted from

  • Renosto, F.; Seubert, P.A.; Knudson, P.; Segel, I.H.
    APS kinase from Penicillium chrysogenum. Dissociation and reassociation of subunits as the basis of the reversible heat inactivation (1985), J. Biol. Chem., 260, 1535-1544.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.25 slow inactivation in dilute solutions, even at low temperatures Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 2,4,6-Trinitrobenzene sulfonate in the presence or absence of ATP-sulfurylase Penicillium chrysogenum
2.7.1.25 diethyldicarbonate in the presence or absence of ATP-sulfurylase Penicillium chrysogenum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.25 Mg2+ activation Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 33000
-
 2 * 33000, gel filtration at 46°C, the enzyme dissociates into two inactive monomers by heating above 42°C Penicillium chrysogenum
2.7.1.25 57000
-
 gel filtration at 22°C Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Penicillium chrysogenum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.25 mycelium
-
 Penicillium chrysogenum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.25 24.4
-
-
 Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r

Subunits

EC Number Subunits Comment Organism
2.7.1.25 dimer 2 * 33000, gel filtration at 46°C, the enzyme dissociates into two inactive monomers by heating above 42°C Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.25 30
-
 assay at Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.25 36
-
 equilibrium between active and inactive enzyme form Penicillium chrysogenum
2.7.1.25 40
-
 reversible inactivation above by subunit dissociation, kinetics, MgATP2- or MgADP- stimulate reactivation Penicillium chrysogenum
2.7.1.25 43
-
 reversible inactivation, t1/2: 1 min Penicillium chrysogenum
2.7.1.25 50
-
 1 min, 80% reversible inactivation Penicillium chrysogenum
2.7.1.25 60 100 1 min, 85% reversible inactivation Penicillium chrysogenum
2.7.1.25 80
-
 irreversible inactivation, t1/2: 47 min, pH 8, 0.023 mg protein/ml Penicillium chrysogenum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.25 6
-
 below, reversible inactivation at 30°C, reactivation rate increases with increasing pH Penicillium chrysogenum
2.7.1.25 7
-
 reversible inactivation at 42°C Penicillium chrysogenum