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Literature summary extracted from

  • Hommes, F.A.; Moss, L.; Touchton, J.
    Purification and some properties of liver adenylylsulfate kinase (1987), Biochim. Biophys. Acta, 924, 270-275.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.25 dithiothreitol activation Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.1.25 stable to ammonium sulfate precipitation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 adenosine 5'-phosphosulfate potent substrate inhibition, ATP reverses Rattus norvegicus
2.7.1.25 iodoacetamide strong inhibition, dithiothreitol partially protects Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.25 additional information
-
 additional information kinetic study Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.25 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 14500
-
 4 * 14500, SDS-PAGE Rattus norvegicus
2.7.1.25 58000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 alumina C-gamma gel, ammonium sulfate, hydroxyapatite, Cellex CM, unstable upon column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.25 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.25 52.5
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Rattus norvegicus ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r

Subunits

EC Number Subunits Comment Organism
2.7.1.25 tetramer 4 * 14500, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.25 8.5
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.25 7.5 9 approx. half-maximal activity at pH 7.5, approx. 80% of maximal activity at pH 9.0 Rattus norvegicus