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Literature summary extracted from

  • Gallais, S.; de Crescenzo, M.A.; Laval-Martin, D.L.
    Characterization of soluble calcium calmodulin-dependent and -independent NAD+ kinases from Avena sativa seeds (2001), Aust. J. Plant Physiol., 28, 363-371.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.23 adenine 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
2.7.1.23 AMP 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
2.7.1.23 Caffeine 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
2.7.1.23 Calmodulin a 410000 Da Ca2+-calmodulin-independent isoform and a 63000 Da Ca2+-calmodulin-dependent isoform Avena sativa
2.7.1.23 D-ribose 5-phosphate 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
2.7.1.23 imidazole 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
2.7.1.23 nicotinic acid 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa
2.7.1.23 Pyridine 5 mM, activity of both isoenzymes is modestly enhanced Avena sativa

General Stability

EC Number General Stability Organism
2.7.1.23 partial loss of activity after freezing and thawing Avena sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.23 ADP 5 mM, 4% inhibition of the 410000 Da isoform, 25% inhibition of the 63000 Da isoform Avena sativa
2.7.1.23 chlorpromazin 5 mM, 11% inhibition of the 410000 Da isoform, 80% inhibition of the 63000 Da isoform Avena sativa
2.7.1.23 diphosphate 5 mM, 46% inhibition of the 410000 Da isoform, 69% inhibition of the 63000 Da isoform Avena sativa
2.7.1.23 EGTA 5 mM, 20% inhibition of the 410000 Da isoform, 85% inhibition of the 63000 Da isoform Avena sativa
2.7.1.23 iodoacetic acid 1 mM, 56% inhibition of the 63000 Da isoform, no inhibition of the 410000 Da isoform Avena sativa
2.7.1.23 NEM 1 mM, 92% inhibition of the 63000 Da isoform, 34% inhibition of the 410000 Da isoform Avena sativa
2.7.1.23 R24571 1 mM, 46% inhibition of the 410000 Da isoform, 89% inhibition of the 63000 da isoform Avena sativa
2.7.1.23 Trifluoperazine 2 mM, 8% inhibition of the 410000 da isoform, 83% inhibition of the 63000 Da isoform Avena sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.23 Ca2+ a 410000 Da Ca2+-calmodulin-independent isoform and a 63000 Da Ca2+-calmodulin-dependent isoform Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.23 63000
-
 Ca2+-calmodulin-dependent isoenzyme Avena sativa
2.7.1.23 410000
-
 Ca2+-calmodulin-independent NAD+ kinase, gel filtration Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.23 ATP + NAD+ Avena sativa the 410000 Da isoenzyme could be a housekeeping enzyme, the 63000 Da isoenzyme could be mainly involved in the adaption and response of Avena sativa to environmental signals or stress through changes of redox potential and/or calcium signalling pathways ADP + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Avena sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.23
-
 Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.23 seed culture of after-ripened and dormant seeds Avena sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.23 0.0009966
-
-
 Avena sativa

Storage Stability

EC Number Storage Stability Organism
2.7.1.23 -80°C, 30% v/v glycerol, stable for more than 3 months Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+ the 410000 Da isoenzyme could be a housekeeping enzyme, the 63000 Da isoenzyme could be mainly involved in the adaption and response of Avena sativa to environmental signals or stress through changes of redox potential and/or calcium signalling pathways Avena sativa ADP + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.23 7.5 8 410000 Da Ca2+-calmodulin-independent isoform and 63000 Da Ca2+-calmodulin-dependent isoform Avena sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.23 6.8 9.2 90% of maximal activity between pH 6.8 and pH 9.2, 410000 Da Ca2+-calmodulin-independent isoform Avena sativa
2.7.1.23 7.2 8.5 90% of maximal activity between pH 7.2 and pH 8.5, 63000 Da Ca2+-calmodulin-dependent isoform Avena sativa