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Literature summary extracted from

  • Kurnasov, O.V.; Polanuyer, B.M.; Ananta, S.; Sloutsky, R.; Tam, A.; Gerdes, S.Y.; Osterman, A.L.
    Ribosylnicotinamide kinase domain of NadR protein: identification and implications in NAD biosynthesis (2002), J. Bacteriol., 184, 6906-6917.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.22 expression in Escherichia coli Salmonella enterica
2.7.1.22 expression in Escherichia coli Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.22 0.14
-
 nicotinamide mononucleotide pH 7.5, 37°C, nicotinamide mononucleotide adenylyltransferase activity Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.22 ATP + N-ribosylnicotinamide Escherichia coli involved in NAD salvage pathway ADP + nicotinamide ribonucleotide
-
 ?
2.7.1.22 ATP + N-ribosylnicotinamide Salmonella enterica involved in NAD salvage pathway ADP + nicotinamide ribonucleotide
-
 ?
2.7.1.22 ATP + N-ribosylnicotinamide Haemophilus influenzae involved in NAD salvage pathway ADP + nicotinamide ribonucleotide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.22 Escherichia coli
-
-
-
2.7.1.22 Haemophilus influenzae Q57425
-
-
2.7.1.22 Salmonella enterica
-
 serovar typhimurium LT2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.22 recombinant enzyme, Ni2+-nitrilotriacetic acid-agarose, Superdex 200 Escherichia coli
2.7.1.22 recombinant enzyme, Ni2+-nitrilotriacetic acid-agarose, Superdex 200 Salmonella enterica
2.7.1.22 recombinant enzyme, Ni2+-nitrilotriacetic acid-agarose, Superdex 200 Haemophilus influenzae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.22 ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide enzyme has both ribosylnicotinamide kinase, i.e. EC 2.7.1.22, and nicotinamide mononucleotide adenylyltransferase, i.e. EC 2.7.7.18, activity Escherichia coli
a
2.7.1.22 ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide enzyme has both ribosylnicotinamide kinase, i.e. EC 2.7.1.22, and nicotinamide mononucleotide adenylyltransferase, i.e. EC 2.7.7.18, activity Salmonella enterica
a
2.7.1.22 ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide enzyme has both ribosylnicotinamide kinase, i.e. EC 2.7.1.22, and nicotinamide mononucleotide adenylyltransferase, i.e. EC 2.7.7.18, activity Haemophilus influenzae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.22 0.04
-
-
 Salmonella enterica
2.7.1.22 0.04
-
 pH 7.5, 37°C, nicotinamide mononucleotide adenylyltransferase activity Haemophilus influenzae
2.7.1.22 0.04
-
 pH 7.5, 37°C, ribosylnicotinamide kinase activity Haemophilus influenzae
2.7.1.22 0.9
-
 pH 7.5, 37°C, nicotinamide mononucleotide adenylyltransferase activity Haemophilus influenzae
2.7.1.22 1
-
 pH 7.5, 37°C, ribosylnicotinamide kinase activity Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.22 ATP + N-ribosylnicotinamide
-
 Escherichia coli ADP + nicotinamide ribonucleotide
-
 ?
2.7.1.22 ATP + N-ribosylnicotinamide
-
 Salmonella enterica ADP + nicotinamide ribonucleotide
-
 ?
2.7.1.22 ATP + N-ribosylnicotinamide no activity with N-ribosylnicotinate Haemophilus influenzae ADP + nicotinamide ribonucleotide
-
 ?
2.7.1.22 ATP + N-ribosylnicotinamide involved in NAD salvage pathway Escherichia coli ADP + nicotinamide ribonucleotide
-
 ?
2.7.1.22 ATP + N-ribosylnicotinamide involved in NAD salvage pathway Salmonella enterica ADP + nicotinamide ribonucleotide
-
 ?
2.7.1.22 ATP + N-ribosylnicotinamide involved in NAD salvage pathway Haemophilus influenzae ADP + nicotinamide ribonucleotide
-
 ?
2.7.1.22 ATP + nicotinamide mononucleotide
-
 Escherichia coli NAD+ + diphosphate
-
 r
2.7.1.22 ATP + nicotinamide mononucleotide
-
 Salmonella enterica NAD+ + diphosphate
-
 r
2.7.1.22 ATP + nicotinamide mononucleotide
-
 Haemophilus influenzae NAD+ + diphosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.1.22 NadR
-
 Escherichia coli
2.7.1.22 NadR
-
 Salmonella enterica
2.7.1.22 NadR
-
 Haemophilus influenzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.22 0.92
-
 nicotinamide mononucleotide pH 7.5, 37°C, nicotinamide mononucleotide adenylyltransferase activity Haemophilus influenzae