BRENDA - Enzyme Database show

The phosphorolysis of nicotinamide riboside

Rowen, J.W.; Kornberg, A.; J. Biol. Chem. 193, 497-507 (1951)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.22
0.28
-
phosphate
pH 7.4
Sus scrofa
2.7.1.22
1.1
-
nicotinamide riboside
pH 7.4
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.22
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.22
ammonium sulfate, calcium phosphate gel, alumina gel
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.22
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.22
4.25
-
-
Sus scrofa
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.22
-10°C, several weeks, no loss of activity
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.22
ATP + N-ribosylnicotinamide
-
641173
Sus scrofa
ADP + nicotinamide ribonucleotide
-
641173
Sus scrofa
?
2.7.1.22
phosphate + inosine
-
641173
Sus scrofa
?
-
-
-
?
2.7.1.22
phosphate + nicotinamide riboside
-
641173
Sus scrofa
nicotinamide + ribose-1-phosphate
-
641173
Sus scrofa
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.22
8
-
nicotinamide riboside phosphorolysis
Sus scrofa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.22
6
10
approx. 25% of maximal activity at pH 6.0, approx. 55% of maximal activity at pH 10, nicotinamide riboside phosphorolysis
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.22
0.28
-
phosphate
pH 7.4
Sus scrofa
2.7.1.22
1.1
-
nicotinamide riboside
pH 7.4
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.22
ammonium sulfate, calcium phosphate gel, alumina gel
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.22
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.22
4.25
-
-
Sus scrofa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.22
-10°C, several weeks, no loss of activity
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.22
ATP + N-ribosylnicotinamide
-
641173
Sus scrofa
ADP + nicotinamide ribonucleotide
-
641173
Sus scrofa
?
2.7.1.22
phosphate + inosine
-
641173
Sus scrofa
?
-
-
-
?
2.7.1.22
phosphate + nicotinamide riboside
-
641173
Sus scrofa
nicotinamide + ribose-1-phosphate
-
641173
Sus scrofa
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.22
8
-
nicotinamide riboside phosphorolysis
Sus scrofa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.22
6
10
approx. 25% of maximal activity at pH 6.0, approx. 55% of maximal activity at pH 10, nicotinamide riboside phosphorolysis
Sus scrofa