BRENDA - Enzyme Database show

Human thymidine kinase 2: molecular cloning and characterisation of the enzyme activity with antiviral and cytostatic nucleoside substrates

Wang, L.; Munch-Petersen, B.; Herrstrom Sjoberg, A.; Hellman, U.; Bergman, T.; Jornvall, H.; Eriksson, S.; FEBS Lett. 443, 170-174 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.21
-
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.21
deoxcytidine
poor inhibitor
Homo sapiens
2.7.1.21
thymidine
inhibition of deoxycytidine kinase activity
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
additional information
-
additional information
-
Homo sapiens
2.7.1.21
0.029
-
difluorodeoxyuridine
-
Homo sapiens
2.7.1.21
0.066
-
difluorodeoxycytidine
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.21
Homo sapiens
O00142
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.21
rLyTK1Val-106 and rLyTK1Met-106
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641171
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + arabinosyl cytosine
-
641171
Homo sapiens
?
-
-
-
?
2.7.1.21
ATP + thymidine
ATP can be substituted by CTP
641171
Homo sapiens
ADP + dTMP
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.21
0.006
-
thymidine
-
Homo sapiens
2.7.1.21
0.63
-
deoxycytidine
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.21
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.21
deoxcytidine
poor inhibitor
Homo sapiens
2.7.1.21
thymidine
inhibition of deoxycytidine kinase activity
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.21
0.006
-
thymidine
-
Homo sapiens
2.7.1.21
0.63
-
deoxycytidine
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
additional information
-
additional information
-
Homo sapiens
2.7.1.21
0.029
-
difluorodeoxyuridine
-
Homo sapiens
2.7.1.21
0.066
-
difluorodeoxycytidine
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.21
rLyTK1Val-106 and rLyTK1Met-106
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641171
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + arabinosyl cytosine
-
641171
Homo sapiens
?
-
-
-
?
2.7.1.21
ATP + thymidine
ATP can be substituted by CTP
641171
Homo sapiens
ADP + dTMP
-
-
-
?