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Literature summary extracted from

  • Rechtin, T.M.; Black, M.E.; Mao, F.; Lewis, M.L.; Drake, R.R.
    Purification and photoaffinity labeling of herpes simplex virus type-1 thymidine kinase (1995), J. Biol. Chem., 270, 7055-7060.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21
-
 Herpes simplex virus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.21 C336Y acyclovir resistant, mutation affects ATP binding site Herpes simplex virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 5-azido-2'-deoxyuridine 50% photoincorporation inhibition of wild-type enzyme at 0.003 mM, 50% photoincorporation inhibition of C336Y mutant at 0.05 mM Herpes simplex virus
2.7.1.21 5-azido-2'-deoxyuridine-5'-monophosphate 50% photoincorporation inhibition of wild-type enzyme at 0.05 mM, 50% photoincorporation inhibition of C336Y mutant at 0.135 mM Herpes simplex virus
2.7.1.21 5-bromovinyldeoxyuridine 50% photoincorporation inhibition of wild-type enzyme at 0.0015 mM, 50% photoincorporation inhibition of C336Y mutant above 0.025 mM Herpes simplex virus
2.7.1.21 acyclovir 50% photoincorporation inhibition of wild-type enzyme at 0.05 mM, 50% photoincorporation inhibition of C336Y mutant above 0.3 mM Herpes simplex virus
2.7.1.21 ATP 50% photoincorporation inhibition of wild-type enzyme at 0.15 mM, 50% photoincorporation inhibition of C336Y mutant above 1 mM Herpes simplex virus
2.7.1.21 thymidine 50% photoincorporation inhibition of wild-type enzyme at 0.003 mM, 50% photoincorporation inhibition of C336Y mutant at 0.05 mM Herpes simplex virus
2.7.1.21 thymidylate 50% photoincorporation inhibition of wild-type and C336Y mutant enzyme at 0.0125 mM Herpes simplex virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0013
-
 thymidine pH 7.6, 37°C, wild-type Herpes simplex virus
2.7.1.21 0.0013
-
 ATP pH 7.6, 37°C, mutant C336Y Herpes simplex virus
2.7.1.21 0.01
-
 thymidine pH 7.6, 37°C, mutant C336Y Herpes simplex virus
2.7.1.21 0.03
-
 ATP pH 7.6, 37°C, wild-type Herpes simplex virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Herpes simplex virus
-
 type 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21
-
 Herpes simplex virus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 0.089
-
-
 Herpes simplex virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine
-
 Herpes simplex virus ADP + dTMP
-
 ?