BRENDA - Enzyme Database show

A continuous spectrophotometric assay for thymidine and deoxycytidine kinases

Liu, Y.; Santi, D.V.; Anal. Biochem. 264, 259-262 (1998)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.0047
-
deoxyuridine
pH 7.6
Herpes simplex virus
2.7.1.21
0.013
-
ATP
pH 7.6, with deoxycytidine
Herpes simplex virus
2.7.1.21
0.021
-
ATP
pH 7.6, with deoxyuridine
Herpes simplex virus
2.7.1.21
0.106
-
deoxycytidine
pH 7.6
Herpes simplex virus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.21
Herpes simplex virus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.21
-
Herpes simplex virus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.21
0.46
-
-
Herpes simplex virus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641164
Herpes simplex virus
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + 2'-deoxyuridine
-
641164
Herpes simplex virus
ADP + 2'-deoxyuridine 5'-phosphate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.21
1.3
-
deoxyuridine
pH 7.6
Herpes simplex virus
2.7.1.21
1.4
-
deoxycytidine
pH 7.6
Herpes simplex virus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.0047
-
deoxyuridine
pH 7.6
Herpes simplex virus
2.7.1.21
0.013
-
ATP
pH 7.6, with deoxycytidine
Herpes simplex virus
2.7.1.21
0.021
-
ATP
pH 7.6, with deoxyuridine
Herpes simplex virus
2.7.1.21
0.106
-
deoxycytidine
pH 7.6
Herpes simplex virus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.21
-
Herpes simplex virus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.21
0.46
-
-
Herpes simplex virus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641164
Herpes simplex virus
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + 2'-deoxyuridine
-
641164
Herpes simplex virus
ADP + 2'-deoxyuridine 5'-phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.21
1.3
-
deoxyuridine
pH 7.6
Herpes simplex virus
2.7.1.21
1.4
-
deoxycytidine
pH 7.6
Herpes simplex virus