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Literature summary extracted from

  • Hinds, T.A.; Compadre, C.; Hurlburt, B.K.; Drake, R.R.
    Conservative mutations of glutamine-125 in Herpes simplex virus type 1 thymidine kinase result in a Ganciclovir kinase with minimal deoxypyrimidine kinase activities (2000), Biochemistry, 39, 4105-4111.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21
-
 Herpes simplex virus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.21 Q125D increased Km for thymidine Herpes simplex virus
2.7.1.21 Q125E increased Km for thymidine Herpes simplex virus
2.7.1.21 Q125N increased Km for thymidine and ganciclovir Herpes simplex virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0009
-
 thymidine pH 7.6, 37°C, wild-type Herpes simplex virus
2.7.1.21 0.003
-
 thymidine pH 7.6, 37°C, mutant Q125N Herpes simplex virus
2.7.1.21 0.02
-
 thymidine pH 7.6, 37°C, mutant Q125E Herpes simplex virus
2.7.1.21 0.05
-
 ganciclovir pH 7.6, 37°C, mutant Q125E Herpes simplex virus
2.7.1.21 0.069
-
 ganciclovir pH 7.6, 37°C, wild-type Herpes simplex virus
2.7.1.21 0.473
-
 ganciclovir pH 7.6, 37°C, mutant Q125N Herpes simplex virus
2.7.1.21 6
-
 thymidine pH 7.6, 37°C, mutant Q125D Herpes simplex virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Herpes simplex virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21
-
 Herpes simplex virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + ganciclovir
-
 Herpes simplex virus ?
-
 ?
2.7.1.21 ATP + thymidine
-
 Herpes simplex virus ADP + dTMP
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.21 0.003
-
 thymidine pH 7.6, 37°C, mutant Q125N Herpes simplex virus
2.7.1.21 0.04
-
 ganciclovir pH 7.6, 37°C, mutant Q125N Herpes simplex virus
2.7.1.21 0.06
-
 thymidine pH 7.6, 37°C, wild-type Herpes simplex virus
2.7.1.21 0.08
-
 ganciclovir pH 7.6, 37°C, mutant Q125E Herpes simplex virus
2.7.1.21 0.11
-
 thymidine pH 7.6, 37°C, mutant Q125D Herpes simplex virus
2.7.1.21 0.13
-
 thymidine pH 7.6, 37°C, mutant Q125E Herpes simplex virus
2.7.1.21 0.47
-
 ganciclovir pH 7.6, 37°C, wild-type Herpes simplex virus